TY - JOUR
T1 - The Application of Microfluidic Technologies in Aptamer Selection
AU - Liu, Yang
AU - Wang, Nijia
AU - Chan, Chiu-Wing
AU - Lu, Aiping
AU - Yu, Yuanyuan
AU - Zhang, Ge
AU - Ren, Kangning
N1 - Funding Information:
This work was supported by NSFC (51773173 and 81973288), Theme-based Research Scheme (grant number T12-201/20-R), and General Research Fund (grant number 12301720) of the Research Grants Council of the Hong Kong Special Administrative Region, China, Hong Kong Baptist University (SKLP_1718_P01), and SZSTC (SGDX20190816230207535).
Publisher Copyright:
© Copyright © 2021 Liu, Wang, Chan, Lu, Yu, Zhang and Ren.
PY - 2021/9/17
Y1 - 2021/9/17
N2 - Aptamers are sequences of single-strand oligonucleotides (DNA or RNA)
with potential binding capability to specific target molecules, which
are increasingly used as agents for analysis, diagnosis, and medical
treatment. Aptamers are generated by a selection method named systematic
evolution of ligands by exponential enrichment (SELEX). Numerous SELEX
methods have been developed for aptamer selections. However, the
conventional SELEX methods still suffer from high labor intensity, low
operation efficiency, and low success rate. Thus, the applications of
aptamer with desired properties are limited. With their advantages of
low cost, high speed, and upgraded extent of automation, microfluidic
technologies have become promising tools for rapid and high throughput
aptamer selection. This paper reviews current progresses of such
microfluidic systems for aptamer selection. Comparisons of selection
performances with discussions on principles, structure, operations, as
well as advantages and limitations of various microfluidic-based aptamer
selection methods are provided.
AB - Aptamers are sequences of single-strand oligonucleotides (DNA or RNA)
with potential binding capability to specific target molecules, which
are increasingly used as agents for analysis, diagnosis, and medical
treatment. Aptamers are generated by a selection method named systematic
evolution of ligands by exponential enrichment (SELEX). Numerous SELEX
methods have been developed for aptamer selections. However, the
conventional SELEX methods still suffer from high labor intensity, low
operation efficiency, and low success rate. Thus, the applications of
aptamer with desired properties are limited. With their advantages of
low cost, high speed, and upgraded extent of automation, microfluidic
technologies have become promising tools for rapid and high throughput
aptamer selection. This paper reviews current progresses of such
microfluidic systems for aptamer selection. Comparisons of selection
performances with discussions on principles, structure, operations, as
well as advantages and limitations of various microfluidic-based aptamer
selection methods are provided.
KW - aptamer
KW - microarray
KW - microfluidics
KW - oligonucleotide
KW - SELEX
UR - http://www.scopus.com/inward/record.url?scp=85116344137&partnerID=8YFLogxK
U2 - 10.3389/fcell.2021.730035
DO - 10.3389/fcell.2021.730035
M3 - Review article
AN - SCOPUS:85116344137
SN - 2296-634X
VL - 9
JO - Frontiers in Cell and Developmental Biology
JF - Frontiers in Cell and Developmental Biology
M1 - 730035
ER -