The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo

Hio Wa Lam, Hui Chao Lin, Sin Cheng Lao, Jian Li Gao, Si Jia Hong, Chi Weng Leong, Patrick Y K YUE, Yiu Wa Kwan, Anskar Yu Hung Leung, Yi Tao Wang*, Simon Ming Yuen Lee

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

99 Citations (Scopus)


Angiogenesis plays an important role in a wide range of physiological processes such as wound healing and fetal development. Many diseases are associated with imbalances in regulation of angiogenesis, in which it is either excessive or there is insufficient blood vessel formation. Angelica sinensis (AS), commonly used in the prescriptions of Chinese medicine, is a potential candidate for curing such diseases. However, biological effects of AS on angiogenesis and underlying mechanisms are yet to be fully elucidated. This investigation describes the angiogenic effects of AS extract on human endothelial cells (HUVEC) in vitro and zebrafish in vivo. The extract was demonstrated, by XTT assay and microscopic cell counting, to stimulate the proliferation of HUVEC; in addition, flow cytometry analysis indicated that the extract increased the percentage of HUVEC in the S phase. The wound healing migration assay illustrated that a dramatic increase in migration could be measured in AS extract-treated HUVEC. Meanwhile, the number of invaded cells and the mean tube length were significantly increased in AS extract treatment groups. The extract was also demonstrated to promote changes in subintestinal vessels (SIVs) in zebrafish, one feature of angiogenesis. In addition, AS extract was found by real-time PCR to enhance vascular endothelial growth factor (VEGF) mRNA expression. In a bead-based immunoassay, higher levels of p38 and JN K1/2 expression were also observed in effusions compared with control cells. All results suggest that Angelica sinensis extract can promote angiogenesis, and that the angiogenic effects involve p38 and JNK 1/2 phosphorylation.

Original languageEnglish
Pages (from-to)195-211
Number of pages17
JournalJournal of Cellular Biochemistry
Issue number1
Publication statusPublished - 1 Jan 2008

Scopus Subject Areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

User-Defined Keywords

  • Angelica sinensis
  • Angiogenesis
  • VEGF
  • Zebrafish


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