TY - JOUR
T1 - The Analgesic Effect of Paeoniflorin on Neonatal Maternal Separation–Induced Visceral Hyperalgesia in Rats
AU - Zhang, Xiao jun
AU - Li, Zhi
AU - Leung, Wai mui
AU - Liu, Liang
AU - Xu, Hong xi
AU - Bian, Zhaoxiang
N1 - Funding Information:
Supported by the Hong Kong Jockey Club Institute of Chinese Medicine (JCICM-16-02) and a Faculty Research Grant, Hong Kong Baptist University (FRG /04-05/II-54).
PY - 2008/6
Y1 - 2008/6
N2 - Paeoniflorin (PF) is one of the principle active ingredients of the root of Paeonia lactiflora Pall (family Ranunculaceae), a Chinese herb traditionally used to relieve pain, especially visceral pain. The present study aimed to investigate both the effect of PF on neonatal maternal separation-induced visceral hyperalgesia in rats and the mechanism by which such effect is exerted. A dose-dependent analgesic effect was produced by PF (45, 90, 180, and 360 mg/kg i.p.). Centrally administered PF (4.5 mg/kg i.c.v) also produced a significant analgesic effect. The analgesic effect of PF (45 mg/kg i.p.) was maximal at 30 minutes after administration. Furthermore, it was found that nor-binaltorphimine (nor-BNI, 3 mg/kg i.p.), dl-α-methyltyrosine (α-AMPT, 250 mg/kg i.p.), and yohimbine (3 mg/kg i.p.) could block the analgesic effect of PF (45 mg/kg i.p.). Time course determination of PF in brain nuclei showed that the maximal concentration of PF was 30 minutes after intraperitoneal administration of PF (180 mg/kg) in cerebral nuclei, involving the amygdala, hypothalamus, thalamus, and cortex. These data indicate that PF has an analgesic effect on visceral pain in rats with neonatal maternal separation and that this effect may be mediated by κ-opioid receptors and α2-adrenoceptors in the central nervous system. Perspective: This study demonstrates that PF has an analgesic effect on pain in visceral hyperalgesic rats. These results suggest that PF might be potentially useful in clinical therapy for irritable bowel syndrome as a pharmacological agent in alleviating visceral pain.
AB - Paeoniflorin (PF) is one of the principle active ingredients of the root of Paeonia lactiflora Pall (family Ranunculaceae), a Chinese herb traditionally used to relieve pain, especially visceral pain. The present study aimed to investigate both the effect of PF on neonatal maternal separation-induced visceral hyperalgesia in rats and the mechanism by which such effect is exerted. A dose-dependent analgesic effect was produced by PF (45, 90, 180, and 360 mg/kg i.p.). Centrally administered PF (4.5 mg/kg i.c.v) also produced a significant analgesic effect. The analgesic effect of PF (45 mg/kg i.p.) was maximal at 30 minutes after administration. Furthermore, it was found that nor-binaltorphimine (nor-BNI, 3 mg/kg i.p.), dl-α-methyltyrosine (α-AMPT, 250 mg/kg i.p.), and yohimbine (3 mg/kg i.p.) could block the analgesic effect of PF (45 mg/kg i.p.). Time course determination of PF in brain nuclei showed that the maximal concentration of PF was 30 minutes after intraperitoneal administration of PF (180 mg/kg) in cerebral nuclei, involving the amygdala, hypothalamus, thalamus, and cortex. These data indicate that PF has an analgesic effect on visceral pain in rats with neonatal maternal separation and that this effect may be mediated by κ-opioid receptors and α2-adrenoceptors in the central nervous system. Perspective: This study demonstrates that PF has an analgesic effect on pain in visceral hyperalgesic rats. These results suggest that PF might be potentially useful in clinical therapy for irritable bowel syndrome as a pharmacological agent in alleviating visceral pain.
KW - analgesic effect
KW - neonatal maternal separation
KW - Paeoniflorin
KW - visceral hyperalgesia
UR - http://www.scopus.com/inward/record.url?scp=44249126748&partnerID=8YFLogxK
U2 - 10.1016/j.jpain.2007.12.009
DO - 10.1016/j.jpain.2007.12.009
M3 - Journal article
C2 - 18387856
AN - SCOPUS:44249126748
SN - 1526-5900
VL - 9
SP - 497
EP - 505
JO - Journal of Pain
JF - Journal of Pain
IS - 6
ER -