The activity of transient receptor potential channel C-6 modulates the differentiation of fat cells

Yan Qin Tan, Hiu Yee KWAN, Xiaoqiang Yao, Lai K. Leung*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Previously, the V1-3 isoforms of the transient receptor potential channel (TRP) have been shown to promote or prevent adipocyte differentiation. In the current study, the C isoforms were screened for blocking adipogenesis. The hypothesis that the TRP classic or canonical (TRPC) deters adipocyte differentiation was investigated in 3T3-L1 cells employing the channel-specific activator and antagonist, silencing, and overexpression techniques. Fat accumulation in cells was visualized by Oil Red O staining. Intracellular calcium inflow was estimated by confocal microscopy. A high-fat (HF) feeding study was also performed on C57BL/6J mice to verify the findings in the cell model. Among the 6 C isoforms tested, only TRPC-6 inhibited the differentiation of fat cells. The phytochemical quercetin induced the channel protein expression. Calcium-imaging results also revealed that the flavonoid could trigger calcium inflow. Coadministration of quercetin (1 or 20 mg/kg body weight) in an HF diet prevented TRPC-6 from declining and attenuated phosphorylated (p)-PKB and PI3k, as well as the proliferation of visceral fat cells. The present study illustrated that TRPC-6 activation could perturb adipocyte differentiation. The food f lavonoid quercetin was a TRPC-6 inducer and activator and it could prevent adipogenesis in mice.—Tan, Y. Q., Kwan, H. Y., Yao, X., Leung, L. K. The activity of transient receptor potential channel C-6 modulates the differentiation of fat cells. FASEB J. 33, 6526–6538 (2019). www.fasebj.org.

Original languageEnglish
Pages (from-to)6526-6538
Number of pages13
JournalFASEB Journal
Volume33
Issue number5
DOIs
Publication statusPublished - 1 May 2019

Scopus Subject Areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

User-Defined Keywords

  • adipogenesis
  • calcium signaling
  • quercetin
  • TRPC

Fingerprint

Dive into the research topics of 'The activity of transient receptor potential channel C-6 modulates the differentiation of fat cells'. Together they form a unique fingerprint.

Cite this