TY - JOUR
T1 - Tetra-proline modified calix[4]arene bonded silica gel
T2 - A novel stationary phase for hydrophilic interaction liquid chromatography
AU - Zhang, Wenfen
AU - Zhang, Yanhao
AU - Zhang, Yongming
AU - Lan, Chen
AU - Miao, Ying
AU - Deng, Zhifen
AU - Ba, Xin
AU - Zhao, Wuduo
AU - Zhang, Shusheng
N1 - Funding information:
We acknowledge the support of National Natural Science Foundation of China (21775140, 21475119, 21705143) and Henan Province outstanding scientific and technological innovation talent (184200510019). Thank Dr. Po-Sun Huang for reviewing the manuscript.
Publisher Copyright:
© 2018 Elsevier B.V. All rights reserved.
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Calixarenes and their derivatives have been widely used in chromatographic studies due to unique host-guest recognition properties. However, stationary phases in hydrophilic interaction chromatography associated with them have yet to be studied because of their general hydrophobicity. In this paper, a tetra-proline modified calix[4]arene bonded stationary phase (DTPCSP) was prepared and characterized by FT-IR spectra, elemental analysis, solid state 13C NMR, SEM, EDS and thermogravimetric analysis. The chromatographic performance and retention mechanism of the developed stationary phase were validated in hydrophilic interaction mode and compared with a commercial column using a variety of hydrophilic or hydrophobic compounds including phenols, nucleosides and sulfonamides. The developed stationary phase exhibited better selectivity than conventional calixarene phases, and the retention behaviors of phenols on DTPCSP column were elucidated by the calculation of quantum chemistry using ODS as contrast. All the results indicate that the developed DTPCSP stationary phase can be beneficial for simultaneously separating complex hydrophilic samples with high selectivity.
AB - Calixarenes and their derivatives have been widely used in chromatographic studies due to unique host-guest recognition properties. However, stationary phases in hydrophilic interaction chromatography associated with them have yet to be studied because of their general hydrophobicity. In this paper, a tetra-proline modified calix[4]arene bonded stationary phase (DTPCSP) was prepared and characterized by FT-IR spectra, elemental analysis, solid state 13C NMR, SEM, EDS and thermogravimetric analysis. The chromatographic performance and retention mechanism of the developed stationary phase were validated in hydrophilic interaction mode and compared with a commercial column using a variety of hydrophilic or hydrophobic compounds including phenols, nucleosides and sulfonamides. The developed stationary phase exhibited better selectivity than conventional calixarene phases, and the retention behaviors of phenols on DTPCSP column were elucidated by the calculation of quantum chemistry using ODS as contrast. All the results indicate that the developed DTPCSP stationary phase can be beneficial for simultaneously separating complex hydrophilic samples with high selectivity.
KW - Hydrophilic interaction liquid chromatography
KW - Quantum chemistry calculation
KW - Retention mechanism
KW - Tetra-proline modified calix[4]arene bonded stationary phase
UR - http://www.scopus.com/inward/record.url?scp=85054174337&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2018.09.083
DO - 10.1016/j.talanta.2018.09.083
M3 - Journal article
C2 - 30368298
AN - SCOPUS:85054174337
SN - 0039-9140
VL - 193
SP - 56
EP - 63
JO - Talanta
JF - Talanta
ER -
