Taurine reduction associated with heart dysfunction after real-world PM_2.5 exposure in aged mice

Ambient PM_2.5 has been proved to be an independent risk factor for cardiovascular diseases; however, little information is available on the age-dependent effects of PM_2.5 on the cardiovascular system and the underlying mechanisms following chronic exposure. In this study, multi-aged mice were exposed to PM_2.5 via the newly developed real-ambient PM_2.5 exposure system to investigate age-related effects on the heart after long-term exposure. First, the chemical and physical properties of PM_2.5 used in the exposure system were analyzed. The heart rate of conscious mice was recorded, and results showed that exposure of aged mice to PM_2.5 for 26 weeks significantly increased heart rate. Histological analysis and ELISA assays indicated that aged mice were more sensitive to PM_2.5 exposure in terms of inducing cardiac oxidative stress and inflammation. Furthermore, untargeted metabolomics revealed that taurine was involved with the PM_2.5-induced cardiac dysfunction. The reduced taurine concentration in the heart was examined by LC-MS and imaging mass spectrometry; it may be due to the increased p53 expression level, ROS and inflammatory cytokines. These results emphasize the age-dependent effects of PM_2.5 on the cardiovascular system and suggest that taurine may be the novel cardiac effect target for PM_2.5-induced heart dysfunction in the aged.