TY - JOUR
T1 - Taurine reduction associated with heart dysfunction after real-world PM2.5 exposure in aged mice
AU - Qi, Zenghua
AU - Yang, Chun
AU - Liao, Xiaoliang
AU - Song, Yuanyuan
AU - Zhao, Lifang
AU - Liang, Xiaoping
AU - Su, Yuping
AU - Chen, Zhi Feng
AU - Li, Ruijin
AU - Dong, Chuan
AU - Cai, Zongwei
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China ( 21806025 and 91843301 ), the Natural Science Foundation of Guangdong Province ( 2019A1515011294 ), Science and Technology Planning Project of Guangdong Province ( 2020B1212030008 ), National Key Research and Development Project ( 2019YFC1804604 ).
Publisher Copyright:
© 2021 Elsevier B.V.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/8/15
Y1 - 2021/8/15
N2 - Ambient PM2.5 has been proved to be an independent risk factor for cardiovascular diseases; however, little information is available on the age-dependent effects of PM2.5 on the cardiovascular system and the underlying mechanisms following chronic exposure. In this study, multi-aged mice were exposed to PM2.5 via the newly developed real-ambient PM2.5 exposure system to investigate age-related effects on the heart after long-term exposure. First, the chemical and physical properties of PM2.5 used in the exposure system were analyzed. The heart rate of conscious mice was recorded, and results showed that exposure of aged mice to PM2.5 for 26 weeks significantly increased heart rate. Histological analysis and ELISA assays indicated that aged mice were more sensitive to PM2.5 exposure in terms of inducing cardiac oxidative stress and inflammation. Furthermore, untargeted metabolomics revealed that taurine was involved with the PM2.5-induced cardiac dysfunction. The reduced taurine concentration in the heart was examined by LC-MS and imaging mass spectrometry; it may be due to the increased p53 expression level, ROS and inflammatory cytokines. These results emphasize the age-dependent effects of PM2.5 on the cardiovascular system and suggest that taurine may be the novel cardiac effect target for PM2.5-induced heart dysfunction in the aged.
AB - Ambient PM2.5 has been proved to be an independent risk factor for cardiovascular diseases; however, little information is available on the age-dependent effects of PM2.5 on the cardiovascular system and the underlying mechanisms following chronic exposure. In this study, multi-aged mice were exposed to PM2.5 via the newly developed real-ambient PM2.5 exposure system to investigate age-related effects on the heart after long-term exposure. First, the chemical and physical properties of PM2.5 used in the exposure system were analyzed. The heart rate of conscious mice was recorded, and results showed that exposure of aged mice to PM2.5 for 26 weeks significantly increased heart rate. Histological analysis and ELISA assays indicated that aged mice were more sensitive to PM2.5 exposure in terms of inducing cardiac oxidative stress and inflammation. Furthermore, untargeted metabolomics revealed that taurine was involved with the PM2.5-induced cardiac dysfunction. The reduced taurine concentration in the heart was examined by LC-MS and imaging mass spectrometry; it may be due to the increased p53 expression level, ROS and inflammatory cytokines. These results emphasize the age-dependent effects of PM2.5 on the cardiovascular system and suggest that taurine may be the novel cardiac effect target for PM2.5-induced heart dysfunction in the aged.
KW - Age-dependent effect
KW - Cardiac dysfunction
KW - Non-targeted metabolomics
KW - PM exposure
KW - Taurine
UR - http://www.scopus.com/inward/record.url?scp=85103971414&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2021.146866
DO - 10.1016/j.scitotenv.2021.146866
M3 - Journal article
C2 - 33848856
AN - SCOPUS:85103971414
SN - 0048-9697
VL - 782
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 146866
ER -