Targeted pH switched europium complexes monitoring receptor internalisation in living cells

Jack D. Fradgley; Matthieu Starck; Michel Laget; Emmanuel Bourrier; Elodie Dupuis; Laurent Lamarque; Eric Trinquet; Jurriaan M. Zwier; David Parker

doi:10.1039/d1cc01029h

Targeted pH switched europium complexes monitoring receptor internalisation in living cells

Jack D. Fradgley, Matthieu Starck, Michel Laget, Emmanuel Bourrier, Elodie Dupuis, Laurent Lamarque, Eric Trinquet, Jurriaan M. Zwier, David Parker^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

11 Citations (Scopus)

Abstract

We report the design and evaluation of pH responsive luminescent europium(III) probes that allow conjugation to targeting vectors to monitor receptor internalisation in cells. The approach adopted here can be used to tag proteins selectively and to monitor uptake into more acidic organelles, thereby enhancing the performance of time-resolved internalisation assays that require pH monitoring in real time.

Original language	English
Pages (from-to)	5814-5817
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	47
Early online date	4 May 2021
DOIs	https://doi.org/10.1039/d1cc01029h
Publication status	Published - 14 Jun 2021

Scopus Subject Areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/d1cc01029h

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title = "Targeted pH switched europium complexes monitoring receptor internalisation in living cells",

abstract = "We report the design and evaluation of pH responsive luminescent europium(III) probes that allow conjugation to targeting vectors to monitor receptor internalisation in cells. The approach adopted here can be used to tag proteins selectively and to monitor uptake into more acidic organelles, thereby enhancing the performance of time-resolved internalisation assays that require pH monitoring in real time.",

author = "Fradgley, {Jack D.} and Matthieu Starck and Michel Laget and Emmanuel Bourrier and Elodie Dupuis and Laurent Lamarque and Eric Trinquet and Zwier, {Jurriaan M.} and David Parker",

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AU - Fradgley, Jack D.

AU - Starck, Matthieu

AU - Laget, Michel

AU - Bourrier, Emmanuel

AU - Dupuis, Elodie

AU - Lamarque, Laurent

AU - Trinquet, Eric

AU - Zwier, Jurriaan M.

AU - Parker, David

PY - 2021/6/14

Y1 - 2021/6/14

N2 - We report the design and evaluation of pH responsive luminescent europium(III) probes that allow conjugation to targeting vectors to monitor receptor internalisation in cells. The approach adopted here can be used to tag proteins selectively and to monitor uptake into more acidic organelles, thereby enhancing the performance of time-resolved internalisation assays that require pH monitoring in real time.

AB - We report the design and evaluation of pH responsive luminescent europium(III) probes that allow conjugation to targeting vectors to monitor receptor internalisation in cells. The approach adopted here can be used to tag proteins selectively and to monitor uptake into more acidic organelles, thereby enhancing the performance of time-resolved internalisation assays that require pH monitoring in real time.

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U2 - 10.1039/d1cc01029h

DO - 10.1039/d1cc01029h

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SN - 1359-7345

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SP - 5814

EP - 5817

JO - Chemical Communications

JF - Chemical Communications

IS - 47

ER -

Targeted pH switched europium complexes monitoring receptor internalisation in living cells

Abstract

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