Systems-Based Interactome Analysis for Hematopoiesis Effect of Angelicae sinensis Radix: Regulated Network of Cell Proliferation towards Hemopoiesis

Guang Zheng*, He Zhang, Yun Yang, Ying li Sun, Yan jing Zhang, Ju ping Chen, Ting Hao, Cheng Lu, Hong tao Guo, Ge ZHANG, Dan ping Fan, Xiao juan He, Aiping LYU

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Objective: To explore the molecular-level mechanism on the hematopoiesis effect of Angelicae sinensis Radix (ASR) with systems-based interactome analysis. Methods: This systems-based interactome analysis was designed to enforce the workflow of "ASR (herb)→compound→target protein→internal protein actions→ending regulated protein for hematopoiesis". This workflow was deployed with restrictions on regulated proteins expresses in bone marrow and anemia disease and futher validated with experiments. Results: The hematopoiesis mechanism of ASR might be accomplished through regulating pathways of cell proliferation towards hemopoiesis with cross-talking agents of spleen tyrosine kinase (SYK), Janus kinase 2 (JAK2), and interleukin-2-inducible T-cell kinase (ITK). The hematopoietic function of ASR was also validated by colony-forming assay performed on mice bone marrow cells. As a result, SYK, JAK2 and ITK were activated. Conclusion: This study provides a new approach to systematically study and predict the therapeutic mechanism for ASR based on interactome analysis towards biological process with experimental validations.

Original languageEnglish
Pages (from-to)939-947
Number of pages9
JournalChinese Journal of Integrative Medicine
Volume25
Issue number12
DOIs
Publication statusPublished - 1 Dec 2019

Scopus Subject Areas

  • Complementary and alternative medicine
  • Pharmacology (medical)

User-Defined Keywords

  • Angelicae sinensis Radix
  • cell proliferation
  • cross-talking agents
  • hemopoiesis
  • interactome
  • regulation

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