Synthesis of magnetic nanoparticles with immobilized aminophenylboronic acid for selective capture of glycoproteins

Zi An Lin*, Jiang Nan Zheng, Fen Lin, Lan Zhang, Zongwei CAI, Guo Nan Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

119 Citations (Scopus)

Abstract

Aminophenylboronic acid (APBA)-functionalized magnetic iron oxide nanoparticles (Fe3O4 MNPs) were synthesized for the selective capture of glycoproteins from unfractionated protein mixtures. The morphology, adsorption, and recognition properties of the resultant particles were investigated and uniform size APBA-coated MNPs with a mean diameter of ∼15 nm and high magnetic saturation value of 30.6 emu g-1 were obtained, which endued the adsorbent with a large surface area and convenience of isolation. The selectivity and binding capacity of APBA-coated MNPs were evaluated by using standard glycoproteins (cellulose and ovalbumin) and nonglycoproteins (bovine hemoglobin, bovine serum albumin and lysozyme) as model samples. Adsorption experiments and SDS-PAGE demonstrated that the APBA-coated MNPs had higher binding capacity and selectivity for glycoproteins compared to nonglycoproteins. In addition, the practicability of the as-prepared MNPs was further assessed by specific capture of ovalbumin from an egg white sample.

Original languageEnglish
Pages (from-to)518-524
Number of pages7
JournalJournal of Materials Chemistry
Volume21
Issue number2
DOIs
Publication statusPublished - 14 Jan 2011

Scopus Subject Areas

  • Chemistry(all)
  • Materials Chemistry

Fingerprint

Dive into the research topics of 'Synthesis of magnetic nanoparticles with immobilized aminophenylboronic acid for selective capture of glycoproteins'. Together they form a unique fingerprint.

Cite this