Synthesis and Evaluation of Europium Complexes that Switch on Luminescence in Lysosomes of Living Cells

Matthieu Starck*, Jack D. Fradgley, Robert Pal, Jurriaan M. Zwier, Laurent Lamarque, David Parker*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

18 Citations (Scopus)

Abstract

A set of four luminescent EuIII complexes bearing an extended aryl-alkynylpyridine chromophore has been studied, showing very different pH-dependent behaviour in their absorption and emission spectral response. For two complexes with pKa values of 6.45 and 6.20 in protein-containing solution, the emission lifetime increases very significantly following protonation. By varying the gate time during signal acquisition, the ‘switch-on’ intensity ratio could be optimised, and enhancement factors of between 250 to 1330 were measured between pH 8 and 4. The best-behaved probe showed no significant emission dependence on the concentration of endogenous cations, reductants, and serum albumin. It was examined in live-cell imaging studies to monitor time-dependent lysosomal acidification, for which the increase in observed image brightness due to acidification was a factor of 50 in NIH-3T3 cells.

Original languageEnglish
Pages (from-to)766-777
Number of pages12
JournalChemistry - A European Journal
Volume27
Issue number2
Early online date16 Nov 2020
DOIs
Publication statusPublished - 7 Jan 2021

Scopus Subject Areas

  • Catalysis
  • Organic Chemistry

User-Defined Keywords

  • Cells
  • Europium
  • Luminescence
  • Lysosomes
  • pH

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