Synergistic effects of linderanolide b combined with arbutin, PTU or kojic acid on tyrosinase inhibition

You Cheng Hseu, Kuo Chen Cheng, Yi Chieh Lin, Chung Yi Chen, Hsin Yu Chou, Edmond Dik Lung MA, Chung Hang Leung, Zhi Hong Wen, Hui Min D. Wang*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

21 Citations (Scopus)


Melanin uncontrollable accumulation is a serious social problem to not only women, but also men, and causes pigment over-expression disorders such as freckles, melasma or pigmented acne scars. The synergism is used widely in medication, and the effectiveness makes the drug applications more valuable. Within this experiment, three well-known compounds were chosen: kojic acid, 1-phenyl-2-thiourea (PTU) and arbutin, and they were combined individually with our substance linderanolide B, which is purified from Cinnamomum subavenium. Hence, deciphering the synergistic action of possible whitening agents was the goal of this study. The tyrosinase activity, melanin content, and the combination index (CI) values were observed in B16F10 cells, in addition, the consequences were detected by isobologram analysis. We discovered that certain melanin inhibitors showed synergistic properties when they were combined together to suppress tyrosinase activities. As a result, linderanolide B has a potential synergy on tyrosinase inhibition, and it can be used widely in cosmetic and medication industries.

Original languageEnglish
Pages (from-to)1120-1126
Number of pages7
JournalCurrent Pharmaceutical Biotechnology
Issue number12
Publication statusPublished - 1 Oct 2015

Scopus Subject Areas

  • Biotechnology
  • Pharmaceutical Science

User-Defined Keywords

  • Inhibitor
  • Melanin
  • Synergism
  • Tyrosinase


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