TY - JOUR
T1 - Sustainable rhamnolipids production in the next decade – Advancing with Burkholderia thailandensis as a potent biocatalytic strain
AU - Kumar, Rajat
AU - Barbhuiya, Rahul Islam
AU - Bohra, Varsha
AU - Wong, Jonathan W. C.
AU - Singh, Ashutosh
AU - Kaur, Guneet
N1 - Funding Information:
The authors acknowledge the financial support provided by Natural Sciences and Engineering Research Council of Canada (NSERC-Discovery Grant RGPIN-2021–03628 ) and Innovation and Technology Commission (ITC), Hong Kong (project code ITS/176/18 ).
Publisher Copyright:
© 2023 Elsevier GmbH
PY - 2023/7
Y1 - 2023/7
N2 - Rhamnolipids are one of the most
promising eco-friendly green glycolipids for
bio-replacements of commercially available fossil fuel-based surfactants.
However, the current industrial biotechnology practices cannot meet the
required standards due to the low production yields, expensive biomass
feedstocks, complicated processing, and opportunistic pathogenic nature of the
conventional rhamnolipid producer strains. To overcome these problems, it has
become important to realize non-pathogenic producer substitutes and
high-yielding strategies supporting biomass-based production. We hereby review
the inherent characteristics of Burkholderia
thailandensis E264
which favor its competence towards such sustainable rhamnolipid biosynthesis. The underlying biosynthetic networks of this
species have unveiled unique substrate specificity,
carbon flux control and rhamnolipid congener profile. Acknowledging such
desirable traits, the present review provides critical insights towards
metabolism, regulation, upscaling, and applications of B.
thailandensis rhamnolipids.
Identification of their unique and naturally inducible physiology has proved to
be beneficial for achieving previously unmet redox balance and metabolic flux requirements
in rhamnolipids production. These developments in part are targeted by the
strategic optimization of B. thailandensis valorizing low-cost substrates ranging
from agro-industrial byproducts to next generation (waste) fractions.
Accordingly, safer bioconversions can
propel the industrial rhamnolipids in advanced biorefinery domains to promote
circular economy, reduce carbon footprint and increased applicability as both
social and environment friendly bioproducts.
AB - Rhamnolipids are one of the most
promising eco-friendly green glycolipids for
bio-replacements of commercially available fossil fuel-based surfactants.
However, the current industrial biotechnology practices cannot meet the
required standards due to the low production yields, expensive biomass
feedstocks, complicated processing, and opportunistic pathogenic nature of the
conventional rhamnolipid producer strains. To overcome these problems, it has
become important to realize non-pathogenic producer substitutes and
high-yielding strategies supporting biomass-based production. We hereby review
the inherent characteristics of Burkholderia
thailandensis E264
which favor its competence towards such sustainable rhamnolipid biosynthesis. The underlying biosynthetic networks of this
species have unveiled unique substrate specificity,
carbon flux control and rhamnolipid congener profile. Acknowledging such
desirable traits, the present review provides critical insights towards
metabolism, regulation, upscaling, and applications of B.
thailandensis rhamnolipids.
Identification of their unique and naturally inducible physiology has proved to
be beneficial for achieving previously unmet redox balance and metabolic flux requirements
in rhamnolipids production. These developments in part are targeted by the
strategic optimization of B. thailandensis valorizing low-cost substrates ranging
from agro-industrial byproducts to next generation (waste) fractions.
Accordingly, safer bioconversions can
propel the industrial rhamnolipids in advanced biorefinery domains to promote
circular economy, reduce carbon footprint and increased applicability as both
social and environment friendly bioproducts.
KW - Biosurfactants
KW - Di-rhamnolipids
KW - Quorum sensing
KW - Global regulators
KW - Genetic optimization
KW - Bioprocess development
UR - http://www.scopus.com/inward/record.url?scp=85153110248&partnerID=8YFLogxK
U2 - 10.1016/j.micres.2023.127386
DO - 10.1016/j.micres.2023.127386
M3 - Journal article
C2 - 37094547
AN - SCOPUS:85153110248
SN - 0944-5013
VL - 272
JO - Microbiological Research
JF - Microbiological Research
M1 - 127386
ER -