TY - JOUR
T1 - Suppression of OsMDHAR4 enhances heat tolerance by mediating H2O2-induced stomatal closure in rice plants
AU - Liu, Jianping
AU - Sun, Xinjiao
AU - Xu, Feiyun
AU - Zhang, Yingjiao
AU - Zhang, Qian
AU - Miao, Rui
AU - ZHANG, Jianhua
AU - Liang, Jiansheng
AU - Xu, Weifeng
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant number: 31601232, 31761130073), the Natural Science Foundation of Fujian Province (Grant number: 2017 J05046), China Postdoctoral Science Foundation (Grant number: 2017 M612108) and Research Grant of Fujian Agriculture and Forestry University (Grant number: KXGH17005). The authors would like to thank Prof. Jumin Tu (Zhejiang University) for providing the plasmid for subcellular localization.
Funding Information:
This study was supported by the National Natural Science Foundation of China (Grant number: 31601232, 31761130073), the Natural Science Foundation of Fujian Province (Grant number: 2017 J05046), China Postdoctoral Science Foundation (Grant number: 2017 M612108) and Research Grant of Fujian Agriculture and Forestry University (Grant number: KXGH17005).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Background: Monodehydroascorbate reductase (MDAR or MDHAR), which is responsible for growth, development and stress response in plants, is a key enzyme in the maintenance of the ascorbate acid (AsA) pool through the AsA–glutathione (AsA–GSH) cycle. High temperature affects a broad spectrum of cellular components and metabolism including AsA–GSH cycle in plants. In rice, however, the detailed roles of OsMDHAR4 in resistance against heat stress remains unclear. Results: Here, we report that OsMDHAR4 protein was localized to the chloroplasts. OsMDHAR4 expression was detected in all tissues surveyed and peaked in leaf blade. OsMDHAR4 was responsive to multiple stresses and was relatively strongly induced by heat treatment. In comparison with wild type, the osmdhar4 mutant exhibited improved tolerance to heat stress, whereas OsMDHAR4 overexpression lines exhibited enhanced sensitivity to heat stress. Moreover, we found that suppression of OsMDHAR4 promoted stomatal closure and hydrogen peroxide accumulation, and overexpression of OsMDHAR4 increased stomatal opening and decreased hydrogen peroxide content in rice leaves. Conclusions: Taken together, these results indicated that OsMDHAR4 negatively regulates tolerance to heat stress by mediating H2O2-induced stomatal closure in rice.
AB - Background: Monodehydroascorbate reductase (MDAR or MDHAR), which is responsible for growth, development and stress response in plants, is a key enzyme in the maintenance of the ascorbate acid (AsA) pool through the AsA–glutathione (AsA–GSH) cycle. High temperature affects a broad spectrum of cellular components and metabolism including AsA–GSH cycle in plants. In rice, however, the detailed roles of OsMDHAR4 in resistance against heat stress remains unclear. Results: Here, we report that OsMDHAR4 protein was localized to the chloroplasts. OsMDHAR4 expression was detected in all tissues surveyed and peaked in leaf blade. OsMDHAR4 was responsive to multiple stresses and was relatively strongly induced by heat treatment. In comparison with wild type, the osmdhar4 mutant exhibited improved tolerance to heat stress, whereas OsMDHAR4 overexpression lines exhibited enhanced sensitivity to heat stress. Moreover, we found that suppression of OsMDHAR4 promoted stomatal closure and hydrogen peroxide accumulation, and overexpression of OsMDHAR4 increased stomatal opening and decreased hydrogen peroxide content in rice leaves. Conclusions: Taken together, these results indicated that OsMDHAR4 negatively regulates tolerance to heat stress by mediating H2O2-induced stomatal closure in rice.
KW - Heat tolerance
KW - Hydrogen peroxide
KW - Monodehydroascorbate reductase
KW - OsMDHAR4
KW - Rice
KW - Stomata
UR - http://www.scopus.com/inward/record.url?scp=85049146654&partnerID=8YFLogxK
U2 - 10.1186/s12284-018-0230-5
DO - 10.1186/s12284-018-0230-5
M3 - Journal article
AN - SCOPUS:85049146654
SN - 1939-8425
VL - 11
JO - Rice
JF - Rice
IS - 1
M1 - 38
ER -