Subcellular Localization and Functions of Plant lncRNAs in Drought and Salt Stress Tolerance

Tao Qin, Liming XIONG*

*Corresponding author for this work

Research output: Chapter in book/report/conference proceedingChapterpeer-review

3 Citations (Scopus)

Abstract

Plant lncRNAs are expected to play important roles in various plant processes including response to abiotic stress, although the functions of most plant lncRNAs are still unknown. To investigate these potential functions, integrated approaches that employ genetic, transgenic, and molecular biology methods are required. Here, we describe general methods to study the function of lncRNAs in plant response to drought and salt stresses. First, the expression pattern and subcellular localization of lncRNA are analyzed by GUS staining and RNA fluorescence in situ hybridization (FISH). Then the responses of lncRNA mutant and overexpressing transgenic plants to drought and salt stress treatments are characterized, and their sensitivities to ABA are also assayed. To understand the molecular mechanism of lncRNAs’ function in stress response, transcriptome sequencing (RNA-seq) and real-time quantitative PCR are performed to analyze altered expression of stress-related genes. Finally, proline content and ROS content are measured to reveal the accumulation of osmolytes and second messengers in these plants in response to drought and salt stress treatments.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages173-186
Number of pages14
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1933
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Scopus Subject Areas

  • Molecular Biology
  • Genetics

User-Defined Keywords

  • Drought stress
  • FISH
  • GUS staining
  • Plant lncRNAs
  • Proline content
  • ROS content
  • Salt stress
  • Stress response genes
  • Transcriptome sequencing

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