TY - JOUR
T1 - Subcellular and molecular localization of rare earth elements and structural characterization of yttrium bound chlorophyll a in naturally grown fern Dicranopteris dichotoma
AU - Wei, Zhenggui
AU - Hong, Fashui
AU - Yin, Ming
AU - Li, Huixin
AU - Hu, Feng
AU - Zhao, Guiwen
AU - Wong, Jonathan W. C.
N1 - Funding Information:
This work is supported by NSFC, a person of Ability Recommended Foundation of Nanjing Agricultural University, and Project-sponsored by SRF for ROCS, SEM. The authors also thank Dr. Ye Tao at Beijing Synchrotron Radiation Facility for his kind help in EXAFS determination.
Publisher Copyright:
Copyright © 2004 Elsevier B.V. All rights reserved.
PY - 2005/4
Y1 - 2005/4
N2 - A rare earth element (REE) hyperaccumulator, Dicranopteris dichotoma, sampled from an REE mining area in South-Jiangxi region, was chosen for analysis of 15 REEs at subcellular and molecular levels by inductively coupled plasma-mass spectrometry (ICP-MS). The sum of the concentrations of 15 REEs (ΣREEs) of D. dichotoma leaf was about 0.1% dry mass. Results indicated that the ΣREEs of different subcellular fractions of D. dichotoma leaf were cell walls>organelles>the 'soluble' fraction (i.e., cytosol and vacuoles)>cell membranes. ICP-MS results also showed that REEs existed in chlorophylls and lutein, though REE concentrations in carotene and pheophytin were both lower than the procedural blank levels. The ΣREEs of crude lipopolysaccharide and Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) obtained from D. dichotoma leaf were 1200 and 343 mg/kg, respectively. The extended X-ray absorption fine structure (EXAFS) study of D. dichotoma chlorophyll a indicated that yttrium was bound to the porphyrin ring of chlorophyll a, and one yttrium atom was surrounded by four nitrogen atoms with the average yttrium-nitrogen bond length being 0.236 nm. These data might be useful for understanding of the physiological role of REEs in hyperaccumulator D. dichotoma.
AB - A rare earth element (REE) hyperaccumulator, Dicranopteris dichotoma, sampled from an REE mining area in South-Jiangxi region, was chosen for analysis of 15 REEs at subcellular and molecular levels by inductively coupled plasma-mass spectrometry (ICP-MS). The sum of the concentrations of 15 REEs (ΣREEs) of D. dichotoma leaf was about 0.1% dry mass. Results indicated that the ΣREEs of different subcellular fractions of D. dichotoma leaf were cell walls>organelles>the 'soluble' fraction (i.e., cytosol and vacuoles)>cell membranes. ICP-MS results also showed that REEs existed in chlorophylls and lutein, though REE concentrations in carotene and pheophytin were both lower than the procedural blank levels. The ΣREEs of crude lipopolysaccharide and Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) obtained from D. dichotoma leaf were 1200 and 343 mg/kg, respectively. The extended X-ray absorption fine structure (EXAFS) study of D. dichotoma chlorophyll a indicated that yttrium was bound to the porphyrin ring of chlorophyll a, and one yttrium atom was surrounded by four nitrogen atoms with the average yttrium-nitrogen bond length being 0.236 nm. These data might be useful for understanding of the physiological role of REEs in hyperaccumulator D. dichotoma.
KW - Dicranopteris dichotoma
KW - EXAFS
KW - ICP-MS
KW - Rare earth elements
KW - Subcellular localization
UR - http://www.scopus.com/inward/record.url?scp=14044269587&partnerID=8YFLogxK
U2 - 10.1016/j.microc.2004.07.005
DO - 10.1016/j.microc.2004.07.005
M3 - Article
AN - SCOPUS:14044269587
SN - 0026-265X
VL - 80
SP - 1
EP - 8
JO - Microchemical Journal
JF - Microchemical Journal
IS - 1
ER -