TY - JOUR
T1 - Study on the preparation of molecular imprinted polymer for analysis of N-phenylglycine in human urine
AU - Feng, Lei
AU - Liang, Xianyu
AU - Mao, Xuejin
AU - Wan, Hao
AU - Wu, Yan
AU - Han, Quanbin
N1 - Funding Information:
This work is financially supported by the National Natural Science Foundation of China (31960493), the Science and Technology Project of Shenzhen (JCYJ20160531193812867), and the Key-Area Research and Development Program of Guangdong Province (2020B1111110007).
Publisher Copyright:
© 2021
PY - 2021/10/1
Y1 - 2021/10/1
N2 - N-phenylglycine (NPG) in human urine could be an important biomarker for predicting cancers, but its detection has difficulty due to its low abundance in urine. Herein, we report a molecular imprinted polymer (MIP) method to efficiently recognize NPG in urine. The MIP was prepared by precipitation polymerization, adopting NPG as the template, acrylamide (AM) as functional monomer, trimethylpropane triacrylate (TRIM) as crosslinking agent, and acetonitrile as porogen. The specificity and selectivity of MIP towards NPG in human urine were determined by comparing MIP's adsorption to the NPG and N-crotonylglycine (NTG) under the same conditions. The result β = QMIP-NPG/QMIP-NTG = 4.7 indicated the satisfactory specificity and selectivity. Parameters affecting the extraction efficiency were further optimized. Under the optimum conditions, the linear range, limit of detection, and limit of quantification of NPG were 0.5–100 mg∙L−1, 1.6 × 10−2 mg∙L−1, and 5.5 × 10−2 mg∙L−1, respectively. Recoveries of NPG in human urine were in the range of 84.7–100.0% with RSDS of 3.8–10.8%. The developed method demonstrated superior selectivity to the target analyte, which can be applied to separate and enrich the NPG from urine samples.
AB - N-phenylglycine (NPG) in human urine could be an important biomarker for predicting cancers, but its detection has difficulty due to its low abundance in urine. Herein, we report a molecular imprinted polymer (MIP) method to efficiently recognize NPG in urine. The MIP was prepared by precipitation polymerization, adopting NPG as the template, acrylamide (AM) as functional monomer, trimethylpropane triacrylate (TRIM) as crosslinking agent, and acetonitrile as porogen. The specificity and selectivity of MIP towards NPG in human urine were determined by comparing MIP's adsorption to the NPG and N-crotonylglycine (NTG) under the same conditions. The result β = QMIP-NPG/QMIP-NTG = 4.7 indicated the satisfactory specificity and selectivity. Parameters affecting the extraction efficiency were further optimized. Under the optimum conditions, the linear range, limit of detection, and limit of quantification of NPG were 0.5–100 mg∙L−1, 1.6 × 10−2 mg∙L−1, and 5.5 × 10−2 mg∙L−1, respectively. Recoveries of NPG in human urine were in the range of 84.7–100.0% with RSDS of 3.8–10.8%. The developed method demonstrated superior selectivity to the target analyte, which can be applied to separate and enrich the NPG from urine samples.
KW - Human urine
KW - Molecular imprinted polymer (MIP)
KW - Molecular selective extraction
KW - N-phenylglycine
KW - Ultra-high performance liquid chromatography (UPLC)
UR - http://www.scopus.com/inward/record.url?scp=85115016319&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2021.122918
DO - 10.1016/j.jchromb.2021.122918
M3 - Journal article
C2 - 34537499
AN - SCOPUS:85115016319
SN - 1570-0232
VL - 1182
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
M1 - 122918
ER -
