Study on rolling circle amplification of Ebola virus and fluorescence detection based on graphene oxide

Jia Wen; Weisi Li; Jiaqi Li; Binbin Tao; Yongqian Xu; Hongjuan Li; Aiping LYU; Shiguo Sun

doi:10.1016/j.snb.2016.01.036

Study on rolling circle amplification of Ebola virus and fluorescence detection based on graphene oxide

Jia Wen
, Weisi Li
, Jiaqi Li
, Binbin Tao
, Yongqian Xu
, Hongjuan Li
, Aiping LYU^*
, Shiguo Sun

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

62 Citations (Scopus)

Abstract

A graphene oxide (GO) assisted rolling circle amplification (RCA) platform for Ebola virus (EBOV) detection is developed with simplicity and high sensitivity. In the absence of EBOV gene, no RCA products generated and the fluorescein amidate (FAM) labeled detection probe was adsorbed on the surface of GO, resulting in fluorescence quenching of the FAM. Addition of the EBOV gene allowed RCA to be taken place and the formation of double-stranded DNA (dsDNA) between RCA products and FAM labeled detection probe, leading to desorption of the FAM labeled detection probe from GO surface accompanied fluorescence recovery. EBOV gene can be determined both in aqueous solution and 1% serum solution. The limit of detection was 1.4 pM.

Original language	English
Pages (from-to)	655-659
Number of pages	5
Journal	Sensors and Actuators B: Chemical
Volume	227
DOIs	https://doi.org/10.1016/j.snb.2016.01.036
Publication status	Published - 1 May 2016

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

Ebola virus
Fluorescence
Graphene oxide
Rolling circle amplification

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10.1016/j.snb.2016.01.036

Cite this

@article{c55d73bdd534458f8a666a2c4a7e7a89,

title = "Study on rolling circle amplification of Ebola virus and fluorescence detection based on graphene oxide",

abstract = "A graphene oxide (GO) assisted rolling circle amplification (RCA) platform for Ebola virus (EBOV) detection is developed with simplicity and high sensitivity. In the absence of EBOV gene, no RCA products generated and the fluorescein amidate (FAM) labeled detection probe was adsorbed on the surface of GO, resulting in fluorescence quenching of the FAM. Addition of the EBOV gene allowed RCA to be taken place and the formation of double-stranded DNA (dsDNA) between RCA products and FAM labeled detection probe, leading to desorption of the FAM labeled detection probe from GO surface accompanied fluorescence recovery. EBOV gene can be determined both in aqueous solution and 1\% serum solution. The limit of detection was 1.4 pM.",

keywords = "Ebola virus, Fluorescence, Graphene oxide, Rolling circle amplification",

author = "Jia Wen and Weisi Li and Jiaqi Li and Binbin Tao and Yongqian Xu and Hongjuan Li and Aiping LYU and Shiguo Sun",

note = "Funding Information: This work was financially supported by the Scientific Research Foundation of Northwest A\&F University ( Z111021103 and Z111021107 ), the National Natural Science Foundation of China (No. 21206137 , 21272030 , 201205095 ), Shaanxi Province Science and Technology (No. 2013K12-03-23 ), State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University (No. 2013005 ). ",

year = "2016",

month = may,

day = "1",

doi = "10.1016/j.snb.2016.01.036",

language = "English",

volume = "227",

pages = "655--659",

journal = "Sensors and Actuators B: Chemical",

issn = "0925-4005",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Study on rolling circle amplification of Ebola virus and fluorescence detection based on graphene oxide

AU - Wen, Jia

AU - Li, Weisi

AU - Li, Jiaqi

AU - Tao, Binbin

AU - Xu, Yongqian

AU - Li, Hongjuan

AU - LYU, Aiping

AU - Sun, Shiguo

N1 - Funding Information: This work was financially supported by the Scientific Research Foundation of Northwest A&F University ( Z111021103 and Z111021107 ), the National Natural Science Foundation of China (No. 21206137 , 21272030 , 201205095 ), Shaanxi Province Science and Technology (No. 2013K12-03-23 ), State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University (No. 2013005 ).

PY - 2016/5/1

Y1 - 2016/5/1

N2 - A graphene oxide (GO) assisted rolling circle amplification (RCA) platform for Ebola virus (EBOV) detection is developed with simplicity and high sensitivity. In the absence of EBOV gene, no RCA products generated and the fluorescein amidate (FAM) labeled detection probe was adsorbed on the surface of GO, resulting in fluorescence quenching of the FAM. Addition of the EBOV gene allowed RCA to be taken place and the formation of double-stranded DNA (dsDNA) between RCA products and FAM labeled detection probe, leading to desorption of the FAM labeled detection probe from GO surface accompanied fluorescence recovery. EBOV gene can be determined both in aqueous solution and 1% serum solution. The limit of detection was 1.4 pM.

AB - A graphene oxide (GO) assisted rolling circle amplification (RCA) platform for Ebola virus (EBOV) detection is developed with simplicity and high sensitivity. In the absence of EBOV gene, no RCA products generated and the fluorescein amidate (FAM) labeled detection probe was adsorbed on the surface of GO, resulting in fluorescence quenching of the FAM. Addition of the EBOV gene allowed RCA to be taken place and the formation of double-stranded DNA (dsDNA) between RCA products and FAM labeled detection probe, leading to desorption of the FAM labeled detection probe from GO surface accompanied fluorescence recovery. EBOV gene can be determined both in aqueous solution and 1% serum solution. The limit of detection was 1.4 pM.

KW - Ebola virus

KW - Fluorescence

KW - Graphene oxide

KW - Rolling circle amplification

UR - https://www.scopus.com/pages/publications/84955483253

U2 - 10.1016/j.snb.2016.01.036

DO - 10.1016/j.snb.2016.01.036

M3 - Journal article

AN - SCOPUS:84955483253

SN - 0925-4005

VL - 227

SP - 655

EP - 659

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

Study on rolling circle amplification of Ebola virus and fluorescence detection based on graphene oxide

Abstract

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