Hsp70.1在冬凌草甲素处理的肝癌细胞中的作用研究

Translated title of the contribution: Study of the role of Hsp70.1 in oridonin-treated hepatocarcinoma cells

王辉, 叶燕, 黄树林, 禹志领

Research output: Contribution to journalArticle

Abstract

目的研究Hsp70.1在冬凌草甲素处理后的肝癌细胞HepG2中的表达情况及其与冬凌草甲素抗肿瘤活性的关系。方法采用蛋白印迹法及实时荧光定量PCR技术观察Hsp70.1在冬凌草甲素处理的肝癌细胞HepG2的表达变化, 并采用RNA干扰技术观察抑制Hsp70.1表达后对冬凌草甲素抗肝癌效果的影响。结果冬凌草甲素作用12 h后, HepG2细胞的Hsp70.1蛋白及RNA表达均增加。Hsp70.1短发夹RNA(shRNA)转染抑制Hsp70.1表达后,与对照组相比, Hsp70.1 shRNA转染组、冬凌草甲素处理组或两者联合应用组的HepG2细胞的存活率均明显降低。结论 Hsp70.1可促进HepG2细胞的生存, 对HepG2细胞和冬凌草甲素处理的HepG2细胞具有保护作用。
Translated title of the contributionStudy of the role of Hsp70.1 in oridonin-treated hepatocarcinoma cells
Original languageChinese (Simplified)
Pages (from-to)423-426
Number of pages4
Journal广东药学院学报
Volume27
Issue number4
Publication statusPublished - 2011

User-Defined Keywords

  • 热休克蛋白70.1
  • 冬凌草甲素
  • 肝癌细胞

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