Study of the Aggregation of DNA-Capped Gold Nanoparticles: A Smart and Flexible Aptasensor for Spermine Sensing

Tik Hung Tsoi, Yan Juan Gu, Wai Sum Lo, Wai Ting Wong, Wing Tak Wong*, Chi Fai Ng, Chi Sing LEE, Ka-Leung WONG

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

A smart gold nanoparticle based aptasensor is developed for the sensing of this biomarker in a convenient and fast manner. A comprehensive study was performed to elucidate the driving force of DNA adsorption, different factors’ effects, such as gold nanoparticle size, DNA length, concentration, and working pH towards spermine sensing by using UV/Vis absorption spectroscopy and isothermal titration calorimetry. It was found that the developed aptasensor could detect spermine by two different sensing mechanisms simply by adjusting the DNA concentration without complicated procedures. Good performance in complicated matrices was proven by the satisfactory results obtained in the spike analysis of both artificial urine and clinical urine samples. Such a flexible and smart approach described here would provide a useful tool for the fast sensing of spermine and prostate cancer screening.

Original languageEnglish
Pages (from-to)802-809
Number of pages8
JournalChemPlusChem
Volume82
Issue number5
DOIs
Publication statusPublished - 1 May 2017

Scopus Subject Areas

  • Chemistry(all)

User-Defined Keywords

  • DNA
  • gold
  • nanoparticles
  • spermine
  • urine

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