Study of the Aggregation of DNA-Capped Gold Nanoparticles: A Smart and Flexible Aptasensor for Spermine Sensing

Tik Hung Tsoi; Yan Juan Gu; Wai Sum Lo; Wai Ting Wong; Wing Tak Wong; Chi Fai Ng; Chi Sing Lee; Ka Leung Wong

doi:10.1002/cplu.201700155

Study of the Aggregation of DNA-Capped Gold Nanoparticles: A Smart and Flexible Aptasensor for Spermine Sensing

Tik Hung Tsoi, Yan Juan Gu, Wai Sum Lo, Wai Ting Wong, Wing Tak Wong^*, Chi Fai Ng, Chi Sing Lee, Ka Leung Wong

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

14 Citations (Scopus)

Abstract

A smart gold nanoparticle based aptasensor is developed for the sensing of this biomarker in a convenient and fast manner. A comprehensive study was performed to elucidate the driving force of DNA adsorption, different factors’ effects, such as gold nanoparticle size, DNA length, concentration, and working pH towards spermine sensing by using UV/Vis absorption spectroscopy and isothermal titration calorimetry. It was found that the developed aptasensor could detect spermine by two different sensing mechanisms simply by adjusting the DNA concentration without complicated procedures. Good performance in complicated matrices was proven by the satisfactory results obtained in the spike analysis of both artificial urine and clinical urine samples. Such a flexible and smart approach described here would provide a useful tool for the fast sensing of spermine and prostate cancer screening.

Original language	English
Pages (from-to)	802-809
Number of pages	8
Journal	ChemPlusChem
Volume	82
Issue number	5
DOIs	https://doi.org/10.1002/cplu.201700155
Publication status	Published - 1 May 2017

Scopus Subject Areas

Chemistry(all)

User-Defined Keywords

DNA
gold
nanoparticles
spermine
urine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/cplu.201700155

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title = "Study of the Aggregation of DNA-Capped Gold Nanoparticles: A Smart and Flexible Aptasensor for Spermine Sensing",

abstract = "A smart gold nanoparticle based aptasensor is developed for the sensing of this biomarker in a convenient and fast manner. A comprehensive study was performed to elucidate the driving force of DNA adsorption, different factors{\textquoteright} effects, such as gold nanoparticle size, DNA length, concentration, and working pH towards spermine sensing by using UV/Vis absorption spectroscopy and isothermal titration calorimetry. It was found that the developed aptasensor could detect spermine by two different sensing mechanisms simply by adjusting the DNA concentration without complicated procedures. Good performance in complicated matrices was proven by the satisfactory results obtained in the spike analysis of both artificial urine and clinical urine samples. Such a flexible and smart approach described here would provide a useful tool for the fast sensing of spermine and prostate cancer screening.",

keywords = "DNA, gold, nanoparticles, spermine, urine",

author = "Tsoi, {Tik Hung} and Gu, {Yan Juan} and Lo, {Wai Sum} and Wong, {Wai Ting} and Wong, {Wing Tak} and Ng, {Chi Fai} and Lee, {Chi Sing} and Wong, {Ka Leung}",

note = "Publisher Copyright: {\textcopyright} 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",

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T2 - A Smart and Flexible Aptasensor for Spermine Sensing

AU - Tsoi, Tik Hung

AU - Gu, Yan Juan

AU - Lo, Wai Sum

AU - Wong, Wai Ting

AU - Wong, Wing Tak

AU - Ng, Chi Fai

AU - Lee, Chi Sing

AU - Wong, Ka Leung

PY - 2017/5/1

Y1 - 2017/5/1

N2 - A smart gold nanoparticle based aptasensor is developed for the sensing of this biomarker in a convenient and fast manner. A comprehensive study was performed to elucidate the driving force of DNA adsorption, different factors’ effects, such as gold nanoparticle size, DNA length, concentration, and working pH towards spermine sensing by using UV/Vis absorption spectroscopy and isothermal titration calorimetry. It was found that the developed aptasensor could detect spermine by two different sensing mechanisms simply by adjusting the DNA concentration without complicated procedures. Good performance in complicated matrices was proven by the satisfactory results obtained in the spike analysis of both artificial urine and clinical urine samples. Such a flexible and smart approach described here would provide a useful tool for the fast sensing of spermine and prostate cancer screening.

AB - A smart gold nanoparticle based aptasensor is developed for the sensing of this biomarker in a convenient and fast manner. A comprehensive study was performed to elucidate the driving force of DNA adsorption, different factors’ effects, such as gold nanoparticle size, DNA length, concentration, and working pH towards spermine sensing by using UV/Vis absorption spectroscopy and isothermal titration calorimetry. It was found that the developed aptasensor could detect spermine by two different sensing mechanisms simply by adjusting the DNA concentration without complicated procedures. Good performance in complicated matrices was proven by the satisfactory results obtained in the spike analysis of both artificial urine and clinical urine samples. Such a flexible and smart approach described here would provide a useful tool for the fast sensing of spermine and prostate cancer screening.

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KW - gold

KW - nanoparticles

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KW - urine

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Study of the Aggregation of DNA-Capped Gold Nanoparticles: A Smart and Flexible Aptasensor for Spermine Sensing

Abstract

Scopus Subject Areas

User-Defined Keywords

UN SDGs

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