TY - JOUR
T1 - Study of BDE-47 induced Parkinson's disease-like metabolic changes in C57BL/6 mice by integrated metabolomic, lipidomic and proteomic analysis
AU - Ji, Fenfen
AU - Sreenivasmurthy, Sravan Gopalkrishnashetty
AU - Wei, Juntong
AU - Shao, Xiaojian
AU - Luan, Hemi
AU - Zhu, Lin
AU - Song, Juxian
AU - Liu, Liangfeng
AU - Li, Min
AU - Cai, Zongwei
N1 - Funding Information:
This work was supported by the Hong Kong Research Grants Council ( RGC-CRF C2014-14E , RGC-GRF 12300114 , RGC-GRF 12101417 and RGC-GRF 12100618 ), HKBU Interdisciplinary Research Matching Scheme ( RC-IRMS/15-16/04 and RC-IRCs/17-18/03 ) and National Natural Science Foundation of China ( 2017YFE0191000 ).
PY - 2019/10/15
Y1 - 2019/10/15
N2 - As the predominant congener of polybrominated diphenyl ethers (PBDEs) detected in human serum, 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) has been reported to induce neurotoxicity. However, the possible linkage between BDE-47 and typical neurodegenerative diseases such as Parkinson's disease (PD) is still unclear. Here we carried out omics studies using liquid chromatography-orbitrap mass spectrometry (LC-orbitrap MS) to depict the BDE-47 induced metabolic changes in C57BJ/L mice to explore the possible contribution of BDE-47 exposure to PD pathology. BDE-47 dissolved in corn oil was orally administered to mice for 30 consecutive days. Results of metabolomics and lipidomics studies of PD-related brain regions revealed significant metabolite changes in pathways involved in oxidative stress and neurotransmitter production. Moreover, isobaric tags for relative and absolute quantitation (iTRAQ) proteomics study of the striatum, which is the part of brain that is most intensively studied in PD pathogenesis, revealed that BDE-47 could induce neurotransmitter system disturbance, abnormal phosphorylation, mitochondrial dysfunction and oxidative stress. Overall, this study depicts the possible contribution of BDE-47 exposure to PD pathology and highlights the powerfulness of omics platforms to deepen the mechanistic understanding of environmental pollutant-caused toxicity.
AB - As the predominant congener of polybrominated diphenyl ethers (PBDEs) detected in human serum, 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) has been reported to induce neurotoxicity. However, the possible linkage between BDE-47 and typical neurodegenerative diseases such as Parkinson's disease (PD) is still unclear. Here we carried out omics studies using liquid chromatography-orbitrap mass spectrometry (LC-orbitrap MS) to depict the BDE-47 induced metabolic changes in C57BJ/L mice to explore the possible contribution of BDE-47 exposure to PD pathology. BDE-47 dissolved in corn oil was orally administered to mice for 30 consecutive days. Results of metabolomics and lipidomics studies of PD-related brain regions revealed significant metabolite changes in pathways involved in oxidative stress and neurotransmitter production. Moreover, isobaric tags for relative and absolute quantitation (iTRAQ) proteomics study of the striatum, which is the part of brain that is most intensively studied in PD pathogenesis, revealed that BDE-47 could induce neurotransmitter system disturbance, abnormal phosphorylation, mitochondrial dysfunction and oxidative stress. Overall, this study depicts the possible contribution of BDE-47 exposure to PD pathology and highlights the powerfulness of omics platforms to deepen the mechanistic understanding of environmental pollutant-caused toxicity.
KW - BDE-47
KW - Dopamine
KW - Mass spectrometry
KW - Omics
KW - Parkinson's disease
UR - http://www.scopus.com/inward/record.url?scp=85067181452&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2019.06.015
DO - 10.1016/j.jhazmat.2019.06.015
M3 - Journal article
C2 - 31203119
AN - SCOPUS:85067181452
SN - 0304-3894
VL - 378
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 120738
ER -