TY - JOUR
T1 - Studies on the aconitine-type alkaloids in the roots of Aconitum Carmichaeli Debx. by HPLC/ESIMS/MSn
AU - Yue, Hao
AU - Pi, Zifeng
AU - Song, Fengrui
AU - Liu, Zhiqiang
AU - CAI, Zongwei
AU - Liu, Shuying
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (No. 30472134, 30672600) and 973 project (No. 2006CB5047060). The authors gratefully acknowledge Prof. F.P. Wang and D.L. Chen (West China College of Pharmacy, Sichuan University, China) for their kind help.
PY - 2009/3/15
Y1 - 2009/3/15
N2 - Studies of aconitine-type alkaloids in the Chinese herb Aconitum Carmichaeli were performed by HPLC/ESIMS/MSn and FTICR/ESIMS in positive ion mode. The characteristic fragmentation pathways in the MSn spectra were summarized based on previously published research literature and further study. According to the fragmentation pathways of mass spectrometry, results from the analysis of standard compounds and reports from literature, 111 compounds were identified or deduced in a total of 117 found compounds in A. Carmichaeli. In the 11 monoester-diterpenoid alkaloids (MDA), 10 diesterditerpenoid alkaloids (DDA) and 81 lipo-alkaloids, the novel alkaloids including 1 MDA, 2 DDA and 48 lipo-alkaloids were detected. In addition, 1 DDA, 7 lipo-alkaloids and 2 alkaloids with small molecular weights that possess C19-norditerpenoid skeleton were reported in A. Carmichaeli for the first time.
AB - Studies of aconitine-type alkaloids in the Chinese herb Aconitum Carmichaeli were performed by HPLC/ESIMS/MSn and FTICR/ESIMS in positive ion mode. The characteristic fragmentation pathways in the MSn spectra were summarized based on previously published research literature and further study. According to the fragmentation pathways of mass spectrometry, results from the analysis of standard compounds and reports from literature, 111 compounds were identified or deduced in a total of 117 found compounds in A. Carmichaeli. In the 11 monoester-diterpenoid alkaloids (MDA), 10 diesterditerpenoid alkaloids (DDA) and 81 lipo-alkaloids, the novel alkaloids including 1 MDA, 2 DDA and 48 lipo-alkaloids were detected. In addition, 1 DDA, 7 lipo-alkaloids and 2 alkaloids with small molecular weights that possess C19-norditerpenoid skeleton were reported in A. Carmichaeli for the first time.
KW - Aconitine-type alkaloids
KW - Aconitum Carmichaeli
KW - C-norditerpenoid
KW - FTICR/ESIMS
KW - HPLC/ESIMS/MS
UR - http://www.scopus.com/inward/record.url?scp=58149492552&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2008.10.022
DO - 10.1016/j.talanta.2008.10.022
M3 - Article
C2 - 19159802
AN - SCOPUS:58149492552
SN - 0039-9140
VL - 77
SP - 1800
EP - 1807
JO - Talanta
JF - Talanta
IS - 5
ER -