Studies on the aconitine-type alkaloids in the roots of Aconitum Carmichaeli Debx. by HPLC/ESIMS/MSn

Hao Yue; Zifeng Pi; Fengrui Song; Zhiqiang Liu; Zongwei CAI; Shuying Liu

doi:10.1016/j.talanta.2008.10.022

Studies on the aconitine-type alkaloids in the roots of Aconitum Carmichaeli Debx. by HPLC/ESIMS/MSⁿ

Hao Yue, Zifeng Pi, Fengrui Song, Zhiqiang Liu, Zongwei CAI, Shuying Liu^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

122 Citations (Scopus)

Abstract

Studies of aconitine-type alkaloids in the Chinese herb Aconitum Carmichaeli were performed by HPLC/ESIMS/MSⁿ and FTICR/ESIMS in positive ion mode. The characteristic fragmentation pathways in the MSⁿ spectra were summarized based on previously published research literature and further study. According to the fragmentation pathways of mass spectrometry, results from the analysis of standard compounds and reports from literature, 111 compounds were identified or deduced in a total of 117 found compounds in A. Carmichaeli. In the 11 monoester-diterpenoid alkaloids (MDA), 10 diesterditerpenoid alkaloids (DDA) and 81 lipo-alkaloids, the novel alkaloids including 1 MDA, 2 DDA and 48 lipo-alkaloids were detected. In addition, 1 DDA, 7 lipo-alkaloids and 2 alkaloids with small molecular weights that possess C19-norditerpenoid skeleton were reported in A. Carmichaeli for the first time.

Original language	English
Pages (from-to)	1800-1807
Number of pages	8
Journal	Talanta
Volume	77
Issue number	5
DOIs	https://doi.org/10.1016/j.talanta.2008.10.022
Publication status	Published - 15 Mar 2009

User-Defined Keywords

Aconitine-type alkaloids
Aconitum Carmichaeli
C-norditerpenoid
FTICR/ESIMS
HPLC/ESIMS/MS

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10.1016/j.talanta.2008.10.022

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title = "Studies on the aconitine-type alkaloids in the roots of Aconitum Carmichaeli Debx. by HPLC/ESIMS/MSn",

abstract = "Studies of aconitine-type alkaloids in the Chinese herb Aconitum Carmichaeli were performed by HPLC/ESIMS/MSn and FTICR/ESIMS in positive ion mode. The characteristic fragmentation pathways in the MSn spectra were summarized based on previously published research literature and further study. According to the fragmentation pathways of mass spectrometry, results from the analysis of standard compounds and reports from literature, 111 compounds were identified or deduced in a total of 117 found compounds in A. Carmichaeli. In the 11 monoester-diterpenoid alkaloids (MDA), 10 diesterditerpenoid alkaloids (DDA) and 81 lipo-alkaloids, the novel alkaloids including 1 MDA, 2 DDA and 48 lipo-alkaloids were detected. In addition, 1 DDA, 7 lipo-alkaloids and 2 alkaloids with small molecular weights that possess C19-norditerpenoid skeleton were reported in A. Carmichaeli for the first time.",

keywords = "Aconitine-type alkaloids, Aconitum Carmichaeli, C-norditerpenoid, FTICR/ESIMS, HPLC/ESIMS/MS",

author = "Hao Yue and Zifeng Pi and Fengrui Song and Zhiqiang Liu and Zongwei CAI and Shuying Liu",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (No. 30472134, 30672600) and 973 project (No. 2006CB5047060). The authors gratefully acknowledge Prof. F.P. Wang and D.L. Chen (West China College of Pharmacy, Sichuan University, China) for their kind help. ",

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AU - Yue, Hao

AU - Pi, Zifeng

AU - Song, Fengrui

AU - Liu, Zhiqiang

AU - CAI, Zongwei

AU - Liu, Shuying

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (No. 30472134, 30672600) and 973 project (No. 2006CB5047060). The authors gratefully acknowledge Prof. F.P. Wang and D.L. Chen (West China College of Pharmacy, Sichuan University, China) for their kind help.

PY - 2009/3/15

Y1 - 2009/3/15

N2 - Studies of aconitine-type alkaloids in the Chinese herb Aconitum Carmichaeli were performed by HPLC/ESIMS/MSn and FTICR/ESIMS in positive ion mode. The characteristic fragmentation pathways in the MSn spectra were summarized based on previously published research literature and further study. According to the fragmentation pathways of mass spectrometry, results from the analysis of standard compounds and reports from literature, 111 compounds were identified or deduced in a total of 117 found compounds in A. Carmichaeli. In the 11 monoester-diterpenoid alkaloids (MDA), 10 diesterditerpenoid alkaloids (DDA) and 81 lipo-alkaloids, the novel alkaloids including 1 MDA, 2 DDA and 48 lipo-alkaloids were detected. In addition, 1 DDA, 7 lipo-alkaloids and 2 alkaloids with small molecular weights that possess C19-norditerpenoid skeleton were reported in A. Carmichaeli for the first time.

AB - Studies of aconitine-type alkaloids in the Chinese herb Aconitum Carmichaeli were performed by HPLC/ESIMS/MSn and FTICR/ESIMS in positive ion mode. The characteristic fragmentation pathways in the MSn spectra were summarized based on previously published research literature and further study. According to the fragmentation pathways of mass spectrometry, results from the analysis of standard compounds and reports from literature, 111 compounds were identified or deduced in a total of 117 found compounds in A. Carmichaeli. In the 11 monoester-diterpenoid alkaloids (MDA), 10 diesterditerpenoid alkaloids (DDA) and 81 lipo-alkaloids, the novel alkaloids including 1 MDA, 2 DDA and 48 lipo-alkaloids were detected. In addition, 1 DDA, 7 lipo-alkaloids and 2 alkaloids with small molecular weights that possess C19-norditerpenoid skeleton were reported in A. Carmichaeli for the first time.

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KW - C-norditerpenoid

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Studies on the aconitine-type alkaloids in the roots of Aconitum Carmichaeli Debx. by HPLC/ESIMS/MSⁿ

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