TY - JOUR
T1 - Structure of a laminarin-type β-(1→3)-glucan from brown algae Sargassum henslowianum and its potential on regulating gut microbiota
AU - Cui, Yongsheng
AU - Zhu, Lixia
AU - Li, Yixuan
AU - Jiang, Siliang
AU - Sun, Qili
AU - Xie, Enyi
AU - Chen, Hubiao
AU - Zhao, Zhongzhen
AU - Qiao, Wei
AU - Xu, Jun
AU - Dong, Caixia
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China ( 81803682 and 81872975 ), Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis ( CTD2018-03 ), and Hong Kong General Research Fund ( 12100219 and 12103919 ).
PY - 2021/3/1
Y1 - 2021/3/1
N2 - A homogeneous polysaccharide named SHNP with apparent molecular weight of 8.4 kDa was purified from brown algae Sargassum henslowianum using ethanol precipitation, ion-exchange chromatography, and gel-filtration column chromatography. Structural analyses reveal that SHNP is completely composed of glucose, and its backbone consists of β-D-(1→3)-Glcp with side chains comprising t-β-D-Glcp attached at the O-6 position. Thus, SHNP is a laminarin-type polysaccharide. In vitro fermentation test results showed that SHNP was digested by gut microbiota; the pH value in the fecal culture of SHNP was significantly decreased; and total short-chain fatty acids, acetic, propionic and n-butyric acids were significantly increased. Furthermore, SHNP regulated the intestinal microbiota composition by stimulating the growth of species belonging to Enterobacteriaceae while depleting Haemophilus parainfluenzae and Gemmiger formicilis. Taken together, these results indicate that SHNP has the potential for regulating gut microbiota, but its specific role in the regulation requires to be further investigated.
AB - A homogeneous polysaccharide named SHNP with apparent molecular weight of 8.4 kDa was purified from brown algae Sargassum henslowianum using ethanol precipitation, ion-exchange chromatography, and gel-filtration column chromatography. Structural analyses reveal that SHNP is completely composed of glucose, and its backbone consists of β-D-(1→3)-Glcp with side chains comprising t-β-D-Glcp attached at the O-6 position. Thus, SHNP is a laminarin-type polysaccharide. In vitro fermentation test results showed that SHNP was digested by gut microbiota; the pH value in the fecal culture of SHNP was significantly decreased; and total short-chain fatty acids, acetic, propionic and n-butyric acids were significantly increased. Furthermore, SHNP regulated the intestinal microbiota composition by stimulating the growth of species belonging to Enterobacteriaceae while depleting Haemophilus parainfluenzae and Gemmiger formicilis. Taken together, these results indicate that SHNP has the potential for regulating gut microbiota, but its specific role in the regulation requires to be further investigated.
KW - Gut microbiota
KW - In vitro fermentation
KW - Sargassum henslowianum
KW - Short-chain fatty acids
KW - β-(1→3)-Glucan
UR - http://www.scopus.com/inward/record.url?scp=85096194398&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2020.117389
DO - 10.1016/j.carbpol.2020.117389
M3 - Journal article
C2 - 33436218
AN - SCOPUS:85096194398
SN - 0144-8617
VL - 255
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 117389
ER -