Structure identification and in vitro anticancer activity of lathyrol-3-phenylacetate-5,15-diacetate

Jian Ye Zhang*, Wen Jing Huang, Hong Mei Sun, Yun Liu, Xiao Qin Zhao, Si Li Tang, Ming Na Sun, Sheng Wang, Jia Jun Li, Ling Ling Zhang, Jun Hua Zhou, Qian Rong Pan, Hubiao CHEN

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Natural products fromthe genus Euphorbia showattention-attracting activities, such as anticancer activity. In this article, classical isolation and structure identification were used in a study on Caper Euphorbia Seed. Subsequently, MTT and wound healing assays, flow cytometry, western blotting, Hoechst 33258 staining and fluorescence microscopy examination were applied to investigate the anticancer activity of the obtained compounds. In a result, lathyrol-3-phenyl- acetate-5,15-diacetate (deoxy Euphorbia factor L1, DEFL1) was isolated from Caper Euphorbia Seed. Moreover, the NMR signals were totally assigned. DEFL1 showed potent inhibition against lung cancer A549 cells, with an IC50 value of 17.51 ± 0.85 μM. Furthermore, DEFL1 suppressed wound healing of A549 cells in a concentration-dependent manner. Mechanically, DEFL1 induced apoptosis, with involvement of an increase of reactive oxygen species (ROS), decrease of mitochondrial membrane potential (δΨm), release of cytochrome c, activity raise of caspase-9 and 3. Characteristic features of apoptosis were observed by fluorescence microscopy. In summary, DEFL1 inhibited growth and induced apoptosis in lung cancer A549 cells via a mitochondrial pathway.

Original languageEnglish
Article number1412
JournalMolecules
Volume22
Issue number9
DOIs
Publication statusPublished - Sep 2017

Scopus Subject Areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

User-Defined Keywords

  • Apoptosis
  • Caper Euphorbia Seed
  • Diterpenoids
  • Lathyrol-3-phenylacetate-5,15-diacetate
  • Lung cancer
  • Natural products

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