Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense

Xiao Qiang Han; Gar Lee Yue; Rui Qi Yue; Cai Xia Dong; Chung Lap Chan; Chun Hay Ko; Wing Shing Cheung; Ke Wang Luo; Hui Dai; Chun Kwok Wong; Ping Chung Leung; Simon Q B HAN

doi:10.1371/journal.pone.0100380

Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense

Xiao Qiang Han, Gar Lee Yue, Rui Qi Yue, Cai Xia Dong, Chung Lap Chan, Chun Hay Ko, Wing Shing Cheung, Ke Wang Luo, Hui Dai, Chun Kwok Wong, Ping Chung Leung, Simon Q B HAN

Chinese Medicine - Teaching and Research Division

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Abstract

A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal- and (1→3)-Glcp side-chains at O-3 position of (1→6)-Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells.

Original language	English
Article number	e100380
Journal	PLoS ONE
Volume	9
Issue number	7
DOIs	https://doi.org/10.1371/journal.pone.0100380
Publication status	Published - 11 Jul 2014

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Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
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title = "Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense",

abstract = "A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal- and (1→3)-Glcp side-chains at O-3 position of (1→6)-Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells.",

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AU - Han, Xiao Qiang

AU - Yue, Gar Lee

AU - Yue, Rui Qi

AU - Dong, Cai Xia

AU - Chan, Chung Lap

AU - Ko, Chun Hay

AU - Cheung, Wing Shing

AU - Luo, Ke Wang

AU - Dai, Hui

AU - Wong, Chun Kwok

AU - Leung, Ping Chung

AU - HAN, Simon Q B

PY - 2014/7/11

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Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense

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