Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense

Xiao Qiang Han, Gar Lee Yue, Rui Qi Yue, Cai Xia Dong, Chung Lap Chan, Chun Hay Ko, Wing Shing Cheung, Ke Wang Luo, Hui Dai, Chun Kwok Wong, Ping Chung Leung, Simon Q B HAN

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal- and (1→3)-Glcp side-chains at O-3 position of (1→6)-Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells.

Original languageEnglish
Article numbere100380
JournalPLoS ONE
Volume9
Issue number7
DOIs
Publication statusPublished - 11 Jul 2014

Scopus Subject Areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint

Dive into the research topics of 'Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense'. Together they form a unique fingerprint.

Cite this