TY - JOUR
T1 - Structure Characterization and Immunomodulating Effects of Polysaccharides Isolated from Dendrobium officinale
AU - Wei, Wei
AU - Feng, Lei
AU - Bao, Wan-Rong
AU - Ma, Dik-Lung
AU - Leung, Chung-Hang
AU - Nie, Shao-Ping
AU - Han, Quan-Bin
N1 - This study was supported by HKSAR Innovation and Technology Fund (ITF), Tier 3, ITS/311/09, and Hong Kong Baptist University (RC-start up grant, MPCF-001-2014/ 2015, and FRG2/14-15/028).
PY - 2016/2
Y1 - 2016/2
N2 - A crude polysaccharide fraction (cDOP) has been determined to be the characteristic marker of Dendrobium officinale, an expensive tea material in Asia, but its chemistry and bioactivity have not been studied. In work reported here, cDOP was destarched (DOP, 90% yield) and separated into two subfraction polysaccharides, DOPa and DOPb, which were characterized by monosaccharide composition and methylation analyses and spectral analyses (FT-IR and 1H and 13C NMR). Both are composed of mannose and glucose at similar ratios and have a similar structure with a backbone of 1,4-linked β-d-mannopyranosyl and β-d-glucopyranosyl residues. Significant differences were observed only in their molecular weights. Bioassay using mouse macrophage cell line RAW264.7 indicated that DOP and its two subfractions enhance cell proliferation, TNF-α secretion, and phagocytosis in a dose-dependent manner. They also induced the proliferation of lymphocytes alone and with mitogens. DOPa and DOPb are thus proven to be major, active polysaccharide markers of D. officinale.
AB - A crude polysaccharide fraction (cDOP) has been determined to be the characteristic marker of Dendrobium officinale, an expensive tea material in Asia, but its chemistry and bioactivity have not been studied. In work reported here, cDOP was destarched (DOP, 90% yield) and separated into two subfraction polysaccharides, DOPa and DOPb, which were characterized by monosaccharide composition and methylation analyses and spectral analyses (FT-IR and 1H and 13C NMR). Both are composed of mannose and glucose at similar ratios and have a similar structure with a backbone of 1,4-linked β-d-mannopyranosyl and β-d-glucopyranosyl residues. Significant differences were observed only in their molecular weights. Bioassay using mouse macrophage cell line RAW264.7 indicated that DOP and its two subfractions enhance cell proliferation, TNF-α secretion, and phagocytosis in a dose-dependent manner. They also induced the proliferation of lymphocytes alone and with mitogens. DOPa and DOPb are thus proven to be major, active polysaccharide markers of D. officinale.
KW - Dendrobium officinale
KW - polysaccharides
KW - chemical structure
KW - RAW264.7 cells
KW - lymphocytes
UR - http://www.scopus.com/inward/record.url?scp=84956905802&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.5b05180
DO - 10.1021/acs.jafc.5b05180
M3 - Journal article
C2 - 26752248
AN - SCOPUS:84956905802
SN - 0021-8561
VL - 64
SP - 881
EP - 889
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 4
ER -