TY - JOUR
T1 - Structural characterization and immuno-modulating activities of a polysaccharide from Ganoderma sinense
AU - Han, Xiao Qiang
AU - Chan, Ben Chung Lap
AU - Yu, Hua
AU - Yang, Yin Hua
AU - Hu, Shui Qing
AU - Ko, Chun Hay
AU - Dong, Cai Xia
AU - Wong, Chun Kwok
AU - Shaw, Pang Chui
AU - Fung, Kwok Pui
AU - Leung, Ping Chung
AU - HSIAO, Wendy W L
AU - Tu, Peng Fei
AU - HAN, Simon Q B
N1 - Funding Information:
This research is funded by the Innovation and Technology Fund ( ITS/311/09 and InP/108/10 ) of the Government of the Hong Kong Special Administrative Region and Hong Kong Baptist University ( FRG2/11-12/048 ). The purchase of the 700 MHz nuclear magnetic spectrometer was by a One-Off Special Equipment Grant ( SEG CUHK08 and CUHK09 ) of the University Grants Council of Hong Kong SAR .
PY - 2012/11
Y1 - 2012/11
N2 - A protein-bound polysaccharide (GSP-4) with a molecular weight of 8.3×105Da, was isolated from the water extract of the fruiting bodies of Ganoderma sinense. Chemical study revealed that this fraction was composed of mannose, glucose and galactose in the molar ratio of 4.7:27.1:1.0, with the sugar residues of t-, 1,3-, 1,4-, 1,6-, 1,3,4- and 1,3,6-linked Glcp, t-linked Galp, and 1,6-linked Manp. The immnomodulatory effects of GSP-4 were assessed using human peripheral blood mononuclear cells (PBMCs) and murine monocyte/macrophage cell line RAW 264.7. We found that GSP-4 could significantly stimulate the production of the immunomodulatory markers tumor necrosis factor α (TNF-α), interleukin (IL)-1β, IL-12, and granulocyte-macrophage colony-stimulating factor (GM-CSF) in PBMCs. This observation was further substantiated in RAW 264.7 cells, as indicated by the increase of nitric oxide (NO), TNF-α and IL-6 production. GSP-4 also enhanced the expression of inducible NO synthase mRNA in dose-dependent manner. Our current finding gives the first piece of evidence to support that GSP-4 possesses some promising immunomodulating effects and it could be a potential candidate to be further used in related cancer immunotherapy.
AB - A protein-bound polysaccharide (GSP-4) with a molecular weight of 8.3×105Da, was isolated from the water extract of the fruiting bodies of Ganoderma sinense. Chemical study revealed that this fraction was composed of mannose, glucose and galactose in the molar ratio of 4.7:27.1:1.0, with the sugar residues of t-, 1,3-, 1,4-, 1,6-, 1,3,4- and 1,3,6-linked Glcp, t-linked Galp, and 1,6-linked Manp. The immnomodulatory effects of GSP-4 were assessed using human peripheral blood mononuclear cells (PBMCs) and murine monocyte/macrophage cell line RAW 264.7. We found that GSP-4 could significantly stimulate the production of the immunomodulatory markers tumor necrosis factor α (TNF-α), interleukin (IL)-1β, IL-12, and granulocyte-macrophage colony-stimulating factor (GM-CSF) in PBMCs. This observation was further substantiated in RAW 264.7 cells, as indicated by the increase of nitric oxide (NO), TNF-α and IL-6 production. GSP-4 also enhanced the expression of inducible NO synthase mRNA in dose-dependent manner. Our current finding gives the first piece of evidence to support that GSP-4 possesses some promising immunomodulating effects and it could be a potential candidate to be further used in related cancer immunotherapy.
KW - Ganoderma sinense
KW - Human peripheral blood mononuclear cells
KW - Immunological activities
KW - Macrophages
KW - Polysaccharide
UR - http://www.scopus.com/inward/record.url?scp=84865551061&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2012.06.029
DO - 10.1016/j.ijbiomac.2012.06.029
M3 - Journal article
C2 - 22750578
AN - SCOPUS:84865551061
SN - 0141-8130
VL - 51
SP - 597
EP - 603
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
IS - 4
ER -
