TY - JOUR
T1 - Structural and functional diversity of a culturable bacterial community during the early stages of revegetation near a Pb/Zn smelter in Guangdong, PR China
AU - Zhang, Chong Bang
AU - Huang, Li Nan
AU - Shu, Wen Sheng
AU - QIU, Jianwen
AU - Zhang, Jin Tao
AU - Lan, Chong Yu
N1 - Funding Information:
Financial supports from the Foundation of Scientific and Technological Study of the Ministry of Education, PR China (No. 031280) and the Foundation of Revegetation Engineering of the Pb/Zn smelter are gratefully acknowledged. We also thank Cheng-Jiang Zai for providing helps in specific experiments.
PY - 2007/5/1
Y1 - 2007/5/1
N2 - The land near a Pb/Zn smelter in the vicinity of Shaoguan city of Southern China, contaminated with heavy metals by smelting activities, was revegetated using an indigenous plant (Paulownia fortunei). The structure and function of the soil bacterial community were investigated to examine whether such microbial indexes could be used as indicators of changes in abiotic environment and vegetative parameters. Soil samples were analyzed for bacterial community level physiological profiles (CLPP) and molecular fingerprinting of 16S rRNA genes. The average well color development (AWCD), functional diversity (HF), and carbon substrate richness (RS) were high in the two young revegetated sites (revegetated for 2 and 3 years, respectively), and low in the old revegetated site (revegetated for 5 years). The structural diversity (HS) and ribotype (RT) showed an identical trend. Changes in the culturable bacterial community structure and function were not correlated with the revegetation age or heavy metal content, but were closely correlated with a number of soil physico-chemical and vegetative parameters. Therefore, the results suggest that the structural and functional parameters of soil culturable bacterial community may be used as sensitive indicators of changes in soil physico-chemical and vegetative parameters during the early stage of revegetation in this area that has been chronically contaminated by heavy metals.
AB - The land near a Pb/Zn smelter in the vicinity of Shaoguan city of Southern China, contaminated with heavy metals by smelting activities, was revegetated using an indigenous plant (Paulownia fortunei). The structure and function of the soil bacterial community were investigated to examine whether such microbial indexes could be used as indicators of changes in abiotic environment and vegetative parameters. Soil samples were analyzed for bacterial community level physiological profiles (CLPP) and molecular fingerprinting of 16S rRNA genes. The average well color development (AWCD), functional diversity (HF), and carbon substrate richness (RS) were high in the two young revegetated sites (revegetated for 2 and 3 years, respectively), and low in the old revegetated site (revegetated for 5 years). The structural diversity (HS) and ribotype (RT) showed an identical trend. Changes in the culturable bacterial community structure and function were not correlated with the revegetation age or heavy metal content, but were closely correlated with a number of soil physico-chemical and vegetative parameters. Therefore, the results suggest that the structural and functional parameters of soil culturable bacterial community may be used as sensitive indicators of changes in soil physico-chemical and vegetative parameters during the early stage of revegetation in this area that has been chronically contaminated by heavy metals.
KW - Community level physiological profiles (CLPP)
KW - Heavy metals
KW - Industrial barrens
KW - Physico-chemical properties
KW - Revegetation
KW - Terminal restriction fragment length polymorphism (T-RFLP)
UR - http://www.scopus.com/inward/record.url?scp=33947203904&partnerID=8YFLogxK
U2 - 10.1016/j.ecoleng.2006.09.007
DO - 10.1016/j.ecoleng.2006.09.007
M3 - Journal article
AN - SCOPUS:33947203904
SN - 0925-8574
VL - 30
SP - 16
EP - 26
JO - Ecological Engineering
JF - Ecological Engineering
IS - 1
ER -