Stretch-sensitive switching among different channel sublevels of an endothelial cation channel

Xiaoqiang Yao*, Hiu Yee Kwan, Yu Huang

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

11 Citations (Scopus)


A mechanosensitive Ca2+-permeable cation channel was recorded by patch clamp in isolated rat aortic endothelial cells. A low level of channel activity could be observed after seal formation. The channel displayed some inward rectification and had a conductance for inward current of approx. 32 pS in Ca2+-free pipette and bath solutions. Negative suction of -10 to -20 mmHg increased the probability of the channel being open. When the negative pressure in the pipette was raised to -35 to -45 mmHg, the channel underwent an abrupt transition to a large conductance substate that was interrupted occasionally by two other low conductance levels. Under this condition, the overwhelming majority of openings and closings were between a main level of 83 pS and the closed level. Compared to the 32 pS substate, the 83 pS large conductance substate had shorter mean open and closed times. The two channel substates had similar ionic selectivity and both were sensitive to the inhibition of cGMP and protein kinase G. This is the first demonstration showing that mechanostress can change the single channel conductance level of an ion channel in eukaryotic cells.

Original languageEnglish
Pages (from-to)381-390
Number of pages10
JournalBiochimica et Biophysica Acta - Biomembranes
Issue number2
Publication statusPublished - 2 Apr 2001

Scopus Subject Areas

  • Biophysics
  • Biochemistry
  • Cell Biology

User-Defined Keywords

  • Mechanosensitive cation channel
  • Conductance substate
  • Calcium
  • Protein kinase G
  • Nitric oxide
  • Guanosine monophosphate
  • cyclic


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