Strategies for developing long-lasting therapeutic nucleic acid aptamer targeting circulating protein: The present and the future

Yihao Zhang, Huarui Zhang, Daniel Wing Ho Chan, Yuan Ma, Aiping Lu, Sifan Yu*, Baoting Zhang*, Ge Zhang*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

8 Citations (Scopus)

Abstract

Aptamers are short, single-stranded DNA or RNA oligonucleotide sequences that can bind specific targets. The molecular weight of aptamers (<20 kDa) is lower than the renal filtration threshold (30∼50 kDa), resulting in very short half-lives in vivo, which limit their druggability. The development of long-lasting modification approaches for aptamers can help address the druggability bottleneck of aptamers. This review summarized two distinct kinds of long-lasting modification approaches for aptamers, including macromolecular modification and low-molecular-weight modification. Though it is a current approach to extend the half-life of aptamers, the macromolecular modification approach could limit the space for the dosage increases, thus causing potential compliance concerns due to large molecular weight. As for the other modification approach, the low-molecular-weight modification approach, which uses low molecular weight coupling agents (LMWCAs) to modify aptamers, could greatly increase the proportion of aptamer moiety. However, some LMWCAs could bind to other proteins, causing a decrease in the drug amounts in blood circulation. Given these issues, the outlook for the next generation of long-lasting modification approaches was proposed at the end, including improving the administration method to increase dosage for aptamer drugs modified by macromolecule and developing Artificial intelligence (AI)-based strategies for optimization of LMWCAs.

Original languageEnglish
Article number1048148
Number of pages11
JournalFrontiers in Cell and Developmental Biology
Volume10
DOIs
Publication statusPublished - 1 Nov 2022

Scopus Subject Areas

  • Developmental Biology
  • Cell Biology

User-Defined Keywords

  • aptamer
  • half-life
  • long-lasting modification
  • low molecular weight coupling agent
  • PEGylation

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