Steroidogenic effect of Erxian decoction for relieving menopause via the p-Akt/PKB pathway in vitro and in vivo

Shi Wei Wang, Ho Pan Cheung, Yao Tong, Jia Lu, Tzi Bun Ng, Yan Bo Zhang, Zhang Jin Zhang, Kai Fai Lee, Jenny Ka Wing Lam, Stephen Cho Wing Sze*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Ethnopharmacological relevance
Erxian decoction (EXD), an empirical Chinese medicine formula, is effectively used in the clinical treatment of menopause-related symptoms in China. Previous data from our group show that EXD has steroidogenic effect on natural menopausal Sprague–Dawley-rats (SD-rats) as an animal model of menopause. However, the mechanistic studies on steroidogenic effects of EXD are still inadequate. Hence, the mechanisms of steroidogenic effects of EXD were studied in vitro and in vivo in this study.

Materials and methods
Menopause causes a decline of endocrine function and a series of symptoms. In this study, 16–20-month-old female SD rats with a low serum estradiol level were employed. Their endocrine functions after treatment with EXD (4.1 g/kg) were assessed by determination of their serum estradiol level. Proteins involved in the steroidogenic pathway including StAR, 17βHSD, 3βHSD, aromatase, and activation of phosphorylated Protein Kinase B (p-Akt/PKB), as well as estradiol receptor proteins (ERα & ERβ) after EXD treatment were analyzed. Kinase inhibition assay was conducted to confirm the mechanism of steroidogenic effects of EXD in vitro. MCF-7 and BT-483 cells were used to investigate whether EXD stimulated breast cancer cell proliferation.

Results
Results revealed a significantly ameliorated serum estradiol level, and a significantly increased expression of ovarian aromatase and PKB in the EXD-treated rats. EXD attenuated 17β-estradiol stimulated proliferation of breast cancer cells.

Conclusions
The results obtained from immunoblotting and measurements of serum estradiol level of the present investigation revealed that EXD may relieve the menopausal syndrome through an upregulation of ovarian aromatase and p-PKB expression without stimulating the growth of breast cancer cells.
Original languageEnglish
Pages (from-to)188-195
Number of pages8
JournalJournal of Ethnopharmacology
Volume195
DOIs
Publication statusPublished - 4 Jan 2017
Externally publishedYes

User-Defined Keywords

  • Steroidogenic
  • Erxian decoction
  • Menopause
  • p-PKB
  • Breast cancer

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