statTarget: A streamlined tool for signal drift correction and interpretations of quantitative mass spectrometry-based omics data

Hemi Luan*, Fenfen Ji, Yu Chen, Zongwei Cai*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

135 Citations (Scopus)

Abstract

Large-scale quantitative mass spectrometry-based metabolomics and proteomics study requires the long-term analysis of multiple batches of biological samples, which often accompanied with significant signal drift and various inter- and intra-batch variations. The unwanted variations can lead to poor inter- and intra-day reproducibility, which is a hindrance to discover real significance. The use of quality control samples and data treatment strategies in the quality assurance procedure provides a mechanism to evaluate the quality and remove the analytical variance of the data. The statTarget we developed is a streamlined tool with an easy-to-use graphical user interface and an integrated suite of algorithms specifically developed for the evaluation of data quality and removal of unwanted variations for quantitative mass spectrometry-based omics data. A novel quality control-based random forest signal correction algorithm, which can remove inter- and intra-batch unwanted variations at feature-level was implanted in the statTarget. Our evaluation based on real samples showed the developed algorithm could improve the data precision and statistical accuracy for mass spectrometry-based metabolomics and proteomics data. Additionally, the statTarget offers the streamlined procedures for data imputation, data normalization, univariate analysis, multivariate analysis, and feature selection. To conclude, the statTarget allows user-friendly the improvement of the data precision for uncovering the biologically differences, which largely facilitates quantitative mass spectrometry-based omics data processing and statistical analysis.

Original languageEnglish
Pages (from-to)66-72
Number of pages7
JournalAnalytica Chimica Acta
Volume1036
DOIs
Publication statusPublished - 7 Dec 2018

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Fingerprint

Dive into the research topics of 'statTarget: A streamlined tool for signal drift correction and interpretations of quantitative mass spectrometry-based omics data'. Together they form a unique fingerprint.

Cite this