Spectroscopic studies on the interaction of Safranine T with DNA in β-cyclodextrin and carboxymethyl-β-cyclodextrin

Guomei Zhang; Yuehong Pang; Shaomin Shuang; Chuan Dong; Martin M F CHOI; Diansheng Liu

doi:10.1016/j.jphotochem.2004.06.016

Spectroscopic studies on the interaction of Safranine T with DNA in β-cyclodextrin and carboxymethyl-β-cyclodextrin

Guomei Zhang, Yuehong Pang, Shaomin Shuang^*, Chuan Dong, Martin M F CHOI, Diansheng Liu

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

39 Citations (Scopus)

Abstract

The interaction of Safranine T (ST) with cyclodextrins (CDs) including (β-CD) and carboxymethyl-β-cyclodextrin (CM-β-CD) and DNA in aqueous solution is studied by UV-vis absorption and steady-state fluorescence technique. The interactive model of ST with double stranded DNA has been investigated by means of the inclusive action of β-CD and CM-β-CD. Based on the changes of absorption, fluorescence and resonance light scattering (RLS) spectra, the intrinsic binding constant (K_ap) and the binding site number (n) of ST with DNA and the inclusion complexes ST-CD with DNA are obtained in the case of 20 mmol/L Tris-HCl buffer solution (pH 7.2). According to the experimental results, it can be inferred that the interactive model of dimer ST with DNA is "electrostatic binding", while the monomer ST with DNA is "intercalative binding".

Original language	English
Pages (from-to)	153-158
Number of pages	6
Journal	Journal of Photochemistry and Photobiology A: Chemistry
Volume	169
Issue number	2
DOIs	https://doi.org/10.1016/j.jphotochem.2004.06.016
Publication status	Published - 15 Jan 2005

Scopus Subject Areas

General Chemistry
General Chemical Engineering
General Physics and Astronomy

User-Defined Keywords

Cyclodextrin
DNA
Safranine T
Supramolecular system

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10.1016/j.jphotochem.2004.06.016

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@article{2727a1eace1346b39b8fd3ac48713ced,

title = "Spectroscopic studies on the interaction of Safranine T with DNA in β-cyclodextrin and carboxymethyl-β-cyclodextrin",

abstract = "The interaction of Safranine T (ST) with cyclodextrins (CDs) including (β-CD) and carboxymethyl-β-cyclodextrin (CM-β-CD) and DNA in aqueous solution is studied by UV-vis absorption and steady-state fluorescence technique. The interactive model of ST with double stranded DNA has been investigated by means of the inclusive action of β-CD and CM-β-CD. Based on the changes of absorption, fluorescence and resonance light scattering (RLS) spectra, the intrinsic binding constant (Kap) and the binding site number (n) of ST with DNA and the inclusion complexes ST-CD with DNA are obtained in the case of 20 mmol/L Tris-HCl buffer solution (pH 7.2). According to the experimental results, it can be inferred that the interactive model of dimer ST with DNA is {"}electrostatic binding{"}, while the monomer ST with DNA is {"}intercalative binding{"}.",

keywords = "Cyclodextrin, DNA, Safranine T, Supramolecular system",

author = "Guomei Zhang and Yuehong Pang and Shaomin Shuang and Chuan Dong and CHOI, {Martin M F} and Diansheng Liu",

note = "Funding Information: The work was supported by the National Natural Science Foundation of China (Nos. 20172035 and 20275022) and Shanxi Province. Meanwhile, it was also supported by the Outstanding Youth Teacher Foundation from National Educational Committee of China. ",

year = "2005",

month = jan,

day = "15",

doi = "10.1016/j.jphotochem.2004.06.016",

language = "English",

volume = "169",

pages = "153--158",

journal = "Journal of Photochemistry and Photobiology A: Chemistry",

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T1 - Spectroscopic studies on the interaction of Safranine T with DNA in β-cyclodextrin and carboxymethyl-β-cyclodextrin

AU - Zhang, Guomei

AU - Pang, Yuehong

AU - Shuang, Shaomin

AU - Dong, Chuan

AU - CHOI, Martin M F

AU - Liu, Diansheng

N1 - Funding Information: The work was supported by the National Natural Science Foundation of China (Nos. 20172035 and 20275022) and Shanxi Province. Meanwhile, it was also supported by the Outstanding Youth Teacher Foundation from National Educational Committee of China.

PY - 2005/1/15

Y1 - 2005/1/15

N2 - The interaction of Safranine T (ST) with cyclodextrins (CDs) including (β-CD) and carboxymethyl-β-cyclodextrin (CM-β-CD) and DNA in aqueous solution is studied by UV-vis absorption and steady-state fluorescence technique. The interactive model of ST with double stranded DNA has been investigated by means of the inclusive action of β-CD and CM-β-CD. Based on the changes of absorption, fluorescence and resonance light scattering (RLS) spectra, the intrinsic binding constant (Kap) and the binding site number (n) of ST with DNA and the inclusion complexes ST-CD with DNA are obtained in the case of 20 mmol/L Tris-HCl buffer solution (pH 7.2). According to the experimental results, it can be inferred that the interactive model of dimer ST with DNA is "electrostatic binding", while the monomer ST with DNA is "intercalative binding".

AB - The interaction of Safranine T (ST) with cyclodextrins (CDs) including (β-CD) and carboxymethyl-β-cyclodextrin (CM-β-CD) and DNA in aqueous solution is studied by UV-vis absorption and steady-state fluorescence technique. The interactive model of ST with double stranded DNA has been investigated by means of the inclusive action of β-CD and CM-β-CD. Based on the changes of absorption, fluorescence and resonance light scattering (RLS) spectra, the intrinsic binding constant (Kap) and the binding site number (n) of ST with DNA and the inclusion complexes ST-CD with DNA are obtained in the case of 20 mmol/L Tris-HCl buffer solution (pH 7.2). According to the experimental results, it can be inferred that the interactive model of dimer ST with DNA is "electrostatic binding", while the monomer ST with DNA is "intercalative binding".

KW - Cyclodextrin

KW - DNA

KW - Safranine T

KW - Supramolecular system

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DO - 10.1016/j.jphotochem.2004.06.016

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AN - SCOPUS:11044222468

SN - 1010-6030

VL - 169

SP - 153

EP - 158

JO - Journal of Photochemistry and Photobiology A: Chemistry

JF - Journal of Photochemistry and Photobiology A: Chemistry

IS - 2

ER -

Spectroscopic studies on the interaction of Safranine T with DNA in β-cyclodextrin and carboxymethyl-β-cyclodextrin

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