Spectral imaging in microscopy aids evaluation of the luminescent probe behaviour of europium complexes

Huishan Li; David Parker; Jing Xiang Zhang; Hongguang Li

doi:10.1016/j.jlumin.2023.119852

Spectral imaging in microscopy aids evaluation of the luminescent probe behaviour of europium complexes

Huishan Li, David Parker^*, Jing Xiang Zhang, Hongguang Li^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

3 Citations (Scopus)

Abstract

In a series of structurally related cationic Eu(III) triamide complexes, the analysis of variations in emission lifetime and spectral signature as a function of probe environment has elucidated the nature of changes to probe speciation. The cationic complexes exhibit an intrinsic tendency to localise in cellular nucleoli or the lysosomes, providing key information in the design and evaluation of related targeted probes. Each of the three complexes studied possesses a coordinated water molecule in aqueous media, that is absent in organic solvents or is displaced by the addition of protein. The complex observed in cellulo is probably a protein-bound Eu complex.

Original language	English
Article number	119852
Journal	Journal of Luminescence
Volume	260
DOIs	https://doi.org/10.1016/j.jlumin.2023.119852
Publication status	Published - Aug 2023

User-Defined Keywords

Emissive lanthanide probes
Luminescent probe
Protein-bound Eu complex
Spectral imaging

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10.1016/j.jlumin.2023.119852

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@article{9ceee238e2ad43bab5bbacbbe1774718,

title = "Spectral imaging in microscopy aids evaluation of the luminescent probe behaviour of europium complexes",

abstract = "In a series of structurally related cationic Eu(III) triamide complexes, the analysis of variations in emission lifetime and spectral signature as a function of probe environment has elucidated the nature of changes to probe speciation. The cationic complexes exhibit an intrinsic tendency to localise in cellular nucleoli or the lysosomes, providing key information in the design and evaluation of related targeted probes. Each of the three complexes studied possesses a coordinated water molecule in aqueous media, that is absent in organic solvents or is displaced by the addition of protein. The complex observed in cellulo is probably a protein-bound Eu complex.",

keywords = "Emissive lanthanide probes, Luminescent probe, Protein-bound Eu complex, Spectral imaging",

author = "Huishan Li and David Parker and Zhang, {Jing Xiang} and Hongguang Li",

note = "We thank the Hong Kong Jockey Club Charities Trust for equipment support. And this work was financially supported by the Science and Technology Planning Project of Guangdong Province ( 2021B1212040016 ). Publisher Copyright: {\textcopyright} 2023 Elsevier B.V.",

year = "2023",

month = aug,

doi = "10.1016/j.jlumin.2023.119852",

language = "English",

volume = "260",

journal = "Journal of Luminescence",

issn = "0022-2313",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Spectral imaging in microscopy aids evaluation of the luminescent probe behaviour of europium complexes

AU - Li, Huishan

AU - Parker, David

AU - Zhang, Jing Xiang

AU - Li, Hongguang

N1 - We thank the Hong Kong Jockey Club Charities Trust for equipment support. And this work was financially supported by the Science and Technology Planning Project of Guangdong Province ( 2021B1212040016 ). Publisher Copyright: © 2023 Elsevier B.V.

PY - 2023/8

Y1 - 2023/8

N2 - In a series of structurally related cationic Eu(III) triamide complexes, the analysis of variations in emission lifetime and spectral signature as a function of probe environment has elucidated the nature of changes to probe speciation. The cationic complexes exhibit an intrinsic tendency to localise in cellular nucleoli or the lysosomes, providing key information in the design and evaluation of related targeted probes. Each of the three complexes studied possesses a coordinated water molecule in aqueous media, that is absent in organic solvents or is displaced by the addition of protein. The complex observed in cellulo is probably a protein-bound Eu complex.

AB - In a series of structurally related cationic Eu(III) triamide complexes, the analysis of variations in emission lifetime and spectral signature as a function of probe environment has elucidated the nature of changes to probe speciation. The cationic complexes exhibit an intrinsic tendency to localise in cellular nucleoli or the lysosomes, providing key information in the design and evaluation of related targeted probes. Each of the three complexes studied possesses a coordinated water molecule in aqueous media, that is absent in organic solvents or is displaced by the addition of protein. The complex observed in cellulo is probably a protein-bound Eu complex.

KW - Emissive lanthanide probes

KW - Luminescent probe

KW - Protein-bound Eu complex

KW - Spectral imaging

UR - http://www.scopus.com/inward/record.url?scp=85151805871&partnerID=8YFLogxK

UR - https://www.sciencedirect.com/science/article/abs/pii/S0022231323001850?via%3Dihub

U2 - 10.1016/j.jlumin.2023.119852

DO - 10.1016/j.jlumin.2023.119852

M3 - Journal article

AN - SCOPUS:85151805871

SN - 0022-2313

VL - 260

JO - Journal of Luminescence

JF - Journal of Luminescence

M1 - 119852

ER -

Spectral imaging in microscopy aids evaluation of the luminescent probe behaviour of europium complexes

Abstract

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