TY - JOUR
T1 - Spatially Resolved Metabolomics and Lipidomics Reveal Salinity and Drought-Tolerant Mechanisms of Cottonseeds
AU - Liu, Bingbing
AU - Wang, Xiaoxiao
AU - Li, Kun
AU - Cai, Zongwei
N1 - This work was supported by the grant from Shenzhen Science and Technology Innovation Commission (SGDX20190816230207535). The authors thank Dr. Jianing Wang at the Department of Chemistry, Hong Kong Baptist University for his input in the experimental methodology and reviewing the manuscript and Dr. Simon Wang at the Language Centre, Hong Kong Baptist University for helping to improve the linguistic presentation of the manuscript. Z.C. would also like to thank the Chair Professor Program of Henan Province and the project of SYL20020103.
Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/7/21
Y1 - 2021/7/21
N2 - In the current era of global climate change, environmental stresses, especially drought and salt, have impaired the growth and productivity of crops, e.g., cotton. Understanding the mechanisms of plants' adaptation to these abiotic stresses is crucial to breed stress-tolerant crop species. In the present study, integrated metabolomics, lipidomics, and mass spectrometry imaging (MSI) were used to discover the spatial distribution of differential metabolites and lipids in two cottonseed cultivars with contrasting drought and salt tolerance properties. Seventeen differential metabolites and 125 differential lipids were identified. Their possible roles in augmenting stress tolerance were illustrated, which were involved in reactive oxygen species scavenging, osmotic adjustment, and cell membrane structure reconstruction. MSI analysis provided a visualization of nine differential lipids and four differential metabolites in cottonseeds with varied abundances and distributions. The results may help understand cottonseeds' convictive metabolic and lipidomic regulatory networks in coping with salinity and drought stresses and give new insights into the stress-tolerance traits relevant to other crops.
AB - In the current era of global climate change, environmental stresses, especially drought and salt, have impaired the growth and productivity of crops, e.g., cotton. Understanding the mechanisms of plants' adaptation to these abiotic stresses is crucial to breed stress-tolerant crop species. In the present study, integrated metabolomics, lipidomics, and mass spectrometry imaging (MSI) were used to discover the spatial distribution of differential metabolites and lipids in two cottonseed cultivars with contrasting drought and salt tolerance properties. Seventeen differential metabolites and 125 differential lipids were identified. Their possible roles in augmenting stress tolerance were illustrated, which were involved in reactive oxygen species scavenging, osmotic adjustment, and cell membrane structure reconstruction. MSI analysis provided a visualization of nine differential lipids and four differential metabolites in cottonseeds with varied abundances and distributions. The results may help understand cottonseeds' convictive metabolic and lipidomic regulatory networks in coping with salinity and drought stresses and give new insights into the stress-tolerance traits relevant to other crops.
KW - cottonseeds
KW - drought tolerance
KW - lipidomics
KW - mass spectrometry imaging
KW - metabolomics
KW - salinity tolerance
UR - http://www.scopus.com/inward/record.url?scp=85111207334&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.1c01598
DO - 10.1021/acs.jafc.1c01598
M3 - Journal article
C2 - 34253015
AN - SCOPUS:85111207334
SN - 0021-8561
VL - 69
SP - 8028
EP - 8037
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 28
ER -
