Spatial Lipidomics Reveals Lipid Changes in the Cotyledon and Plumule of Mung Bean Seeds during Germination

Peisi Xie, Jing Chen, Pengfei Wu, Zongwei Cai*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

1 Citation (Scopus)

Abstract

Seed germination is a vital process in plant development involving dynamic biochemical transformations such as lipid metabolism. However, the spatial distribution and dynamic changes of lipids in different seed compartments during germination are poorly understood. In this study, we employed liquid chromatography/mass spectrometry (LC/MS)-based lipidomics and MALDI mass spectrometry imaging (MSI) to investigate lipid changes occurring in the cotyledon and plumule of mung bean seeds during germination. Lipidomic data revealed that the germination process reduced the levels of many glycerolipids (e.g., triglyceride) and phosphatidylglycerols (e.g., phosphatidylcholine) while increased the levels of lysophospholipids (e.g., lysophosphatidylcholine) in both the cotyledon and plumule. Sphingolipids (e.g., sphingomyelin) displayed altered levels solely in the plumule. Sterol levels increased in the cotyledon but decreased in the plumule. Further imaging results revealed that MALDI–MSI could serve as a supplement and validate LC–MS data. These findings enhance our understanding of the metabolic processes underlying seedling development, with potential implications for crop improvement and seed quality control.
Original languageEnglish
Pages (from-to)19879–19887
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume71
Issue number49
Early online date29 Nov 2023
DOIs
Publication statusPublished - 13 Dec 2023

Scopus Subject Areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

User-Defined Keywords

  • cotyledon and plumule
  • germination
  • lipidomics
  • mass spectrometry imaging
  • mung bean seeds

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