Small Molecule Pin1 Inhibitor Blocking NF-κB Signaling in Prostate Cancer Cells

Ke Jia Wu, Hai Jing Zhong, Guanjun Yang, Chun Wu, Jie Min Huang, Guodong Li, Edmond Dik Lung MA*, Chung Hang Leung

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

34 Citations (Scopus)


Prolyl-isomerase 1 (Pin1) is a conserved enzyme that regulates cell processes such as cell cycle progression, transcriptional regulation, and apoptosis. However, overexpression of Pin1 is correlated with a higher probability of prostate tumor recurrence. We utilized a molecular docking technique to identify Pin1 inhibitors from a database of natural product and natural product-like compounds. The action of the hit compounds against Pin1 activity was studied using multiple methods, including a fluorometric enzymatic assay, co-immunoprecipitation, western blotting, cell thermal shiftm, and other techniques. We have identified compound 1 as a natural-product-like inhibitor of Pin1 activity via structure-based virtual screening and showed that compound 1 could target Pin1 and disrupt the interaction between Pin1 and the p65 subunit of NF-κB in cells. Furthermore, compound 1 reduced nuclear p65 (Thr254) phosphorylation and attenuated NF-κB activity in cells. Finally, compound 1 induced apoptosis in prostate cancer cells. Compound 1 represents a natural product-like Pin1 inhibitor that acts via targeting the Pin1–NF-κB interaction.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalChemistry - An Asian Journal
Issue number3
Publication statusPublished - 2 Feb 2018

Scopus Subject Areas

  • Biochemistry
  • Organic Chemistry

User-Defined Keywords

  • enzymes
  • natural products
  • Pin1
  • protein-protein interactions
  • virtual screening


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