Site-specific binding of chelerythrine and sanguinarine to single pyrimidine bulges in hairpin DNA

Li Ping Bai; Zongwei CAI; Zhongzhen ZHAO; Kazuhiko Nakatani; Zhi Hong JIANG

doi:10.1007/s00216-008-2302-7

Site-specific binding of chelerythrine and sanguinarine to single pyrimidine bulges in hairpin DNA

Li Ping Bai, Zongwei CAI, Zhongzhen ZHAO, Kazuhiko Nakatani, Zhi Hong JIANG^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

31 Citations (Scopus)

Abstract

Spectrofluorometric titration, electrospray ionization time-of-flight mass spectrometric and UV melting methods were employed to study the binding of chelerythrine and sanguinarine to bulged DNA. The results showed that both alkaloids bind specifically to single pyrimidine (C, T) bulge sites. The ability of sanguinarine to bind to both regular and bulged hairpins was found to be stronger than that of chelerythrine, but the binding selectivity of chelerythrine toward single-base bulges was much larger than that of sanguinarine. [Figure not available: see fulltext.]

Original language	English
Pages (from-to)	709-716
Number of pages	8
Journal	Analytical and Bioanalytical Chemistry
Volume	392
Issue number	4
DOIs	https://doi.org/10.1007/s00216-008-2302-7
Publication status	Published - Oct 2008

Scopus Subject Areas

Analytical Chemistry
Biochemistry

User-Defined Keywords

Bulge DNA
Chelerythrine
ESI-MS
Fluorescence spectrometry
Sanguinarine

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10.1007/s00216-008-2302-7

Cite this

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title = "Site-specific binding of chelerythrine and sanguinarine to single pyrimidine bulges in hairpin DNA",

abstract = "Spectrofluorometric titration, electrospray ionization time-of-flight mass spectrometric and UV melting methods were employed to study the binding of chelerythrine and sanguinarine to bulged DNA. The results showed that both alkaloids bind specifically to single pyrimidine (C, T) bulge sites. The ability of sanguinarine to bind to both regular and bulged hairpins was found to be stronger than that of chelerythrine, but the binding selectivity of chelerythrine toward single-base bulges was much larger than that of sanguinarine. [Figure not available: see fulltext.]",

keywords = "Bulge DNA, Chelerythrine, ESI-MS, Fluorescence spectrometry, Sanguinarine",

author = "Bai, {Li Ping} and Zongwei CAI and Zhongzhen ZHAO and Kazuhiko Nakatani and JIANG, {Zhi Hong}",

note = "Funding Information: Acknowledgements This work was financially supported by the Research Grants Council, the University Grants Committee of Hong Kong (HKBU2455/06M), and the Faculty Research Grant of Hong Kong Baptist University.",

year = "2008",

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doi = "10.1007/s00216-008-2302-7",

language = "English",

volume = "392",

pages = "709--716",

journal = "Analytical and Bioanalytical Chemistry",

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TY - JOUR

T1 - Site-specific binding of chelerythrine and sanguinarine to single pyrimidine bulges in hairpin DNA

AU - Bai, Li Ping

AU - CAI, Zongwei

AU - ZHAO, Zhongzhen

AU - Nakatani, Kazuhiko

AU - JIANG, Zhi Hong

N1 - Funding Information: Acknowledgements This work was financially supported by the Research Grants Council, the University Grants Committee of Hong Kong (HKBU2455/06M), and the Faculty Research Grant of Hong Kong Baptist University.

PY - 2008/10

Y1 - 2008/10

N2 - Spectrofluorometric titration, electrospray ionization time-of-flight mass spectrometric and UV melting methods were employed to study the binding of chelerythrine and sanguinarine to bulged DNA. The results showed that both alkaloids bind specifically to single pyrimidine (C, T) bulge sites. The ability of sanguinarine to bind to both regular and bulged hairpins was found to be stronger than that of chelerythrine, but the binding selectivity of chelerythrine toward single-base bulges was much larger than that of sanguinarine. [Figure not available: see fulltext.]

AB - Spectrofluorometric titration, electrospray ionization time-of-flight mass spectrometric and UV melting methods were employed to study the binding of chelerythrine and sanguinarine to bulged DNA. The results showed that both alkaloids bind specifically to single pyrimidine (C, T) bulge sites. The ability of sanguinarine to bind to both regular and bulged hairpins was found to be stronger than that of chelerythrine, but the binding selectivity of chelerythrine toward single-base bulges was much larger than that of sanguinarine. [Figure not available: see fulltext.]

KW - Bulge DNA

KW - Chelerythrine

KW - ESI-MS

KW - Fluorescence spectrometry

KW - Sanguinarine

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U2 - 10.1007/s00216-008-2302-7

DO - 10.1007/s00216-008-2302-7

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SN - 1618-2642

VL - 392

SP - 709

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JO - Analytical and Bioanalytical Chemistry

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Site-specific binding of chelerythrine and sanguinarine to single pyrimidine bulges in hairpin DNA

Abstract

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