Site-specific binding of chelerythrine and sanguinarine to single pyrimidine bulges in hairpin DNA

Li Ping Bai, Zongwei CAI, Zhongzhen ZHAO, Kazuhiko Nakatani, Zhi Hong JIANG*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Spectrofluorometric titration, electrospray ionization time-of-flight mass spectrometric and UV melting methods were employed to study the binding of chelerythrine and sanguinarine to bulged DNA. The results showed that both alkaloids bind specifically to single pyrimidine (C, T) bulge sites. The ability of sanguinarine to bind to both regular and bulged hairpins was found to be stronger than that of chelerythrine, but the binding selectivity of chelerythrine toward single-base bulges was much larger than that of sanguinarine. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)709-716
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
Volume392
Issue number4
DOIs
Publication statusPublished - Oct 2008

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry

User-Defined Keywords

  • Bulge DNA
  • Chelerythrine
  • ESI-MS
  • Fluorescence spectrometry
  • Sanguinarine

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