Single-cell atlas of domestic pig cerebral cortex and hypothalamus

Jiacheng Zhu; Fang Chen; Lihua Luo; Weiying Wu; Jinxia Dai; Jixing Zhong; Xiumei Lin; Chaochao Chai; Peiwen Ding; Langchao Liang; Shiyou Wang; Xiangning Ding; Yin Chen; Haoyu Wang; Jiaying Qiu; Feiyue Wang; Chengcheng Sun; Yuying Zeng; Jian Fang; Xiaosen Jiang; Ping Liu; Gen Tang; Xin Qiu; Xingliang Zhang; Yetian Ruan; Sanjie Jiang; Jiankang Li; Shida Zhu; Xun Xu; Fang Li; Zhongmin Liu; Gang Cao; Dongsheng Chen

doi:10.1016/j.scib.2021.04.002

Single-cell atlas of domestic pig cerebral cortex and hypothalamus

Jiacheng Zhu
, Fang Chen
, Lihua Luo
, Weiying Wu
, Jinxia Dai
, Jixing Zhong
, Xiumei Lin
, Chaochao Chai
, Peiwen Ding
, Langchao Liang
, Shiyou Wang
, Xiangning Ding
, Yin Chen
, Haoyu Wang
, Jiaying Qiu
, Feiyue Wang
, Chengcheng Sun
, Yuying Zeng
, Jian Fang
, Xiaosen Jiang

Ping Liu, Gen Tang, Xin Qiu, Xingliang Zhang, Yetian Ruan, Sanjie Jiang, Jiankang Li, Shida Zhu, Xun Xu, Fang Li, Zhongmin Liu, Gang Cao^*, Dongsheng Chen^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

26 Citations (Scopus)

Abstract

The brain of the domestic pig (Sus scrofa domesticus) has drawn considerable attention due to its high similarities to that of humans. However, the cellular compositions of the pig brain (PB) remain elusive. Here we investigated the single-nucleus transcriptomic profiles of five regions of the PB (frontal lobe, parietal lobe, temporal lobe, occipital lobe, and hypothalamus) and identified 21 cell subpopulations. The cross-species comparison of mouse and pig hypothalamus revealed the shared and specific gene expression patterns at the single-cell resolution. Furthermore, we identified cell types and molecular pathways closely associated with neurological disorders, bridging the gap between gene mutations and pathogenesis. We reported, to our knowledge, the first single-cell atlas of domestic pig cerebral cortex and hypothalamus combined with a comprehensive analysis across species, providing extensive resources for future research regarding neural science, evolutionary developmental biology, and regenerative medicine.

Original language	English
Pages (from-to)	1448-1461
Number of pages	14
Journal	Science Bulletin
Volume	66
Issue number	14
DOIs	https://doi.org/10.1016/j.scib.2021.04.002
Publication status	Published - 30 Jul 2021

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

Domestic pig
Brain cell atlas
Single-cell transcriptome
Neural science

Access to Document

10.1016/j.scib.2021.04.002

Cite this

@article{5a396c4ed66440efaf4fcbdc069d4623,

title = "Single-cell atlas of domestic pig cerebral cortex and hypothalamus",

abstract = "The brain of the domestic pig (Sus scrofa domesticus) has drawn considerable attention due to its high similarities to that of humans. However, the cellular compositions of the pig brain (PB) remain elusive. Here we investigated the single-nucleus transcriptomic profiles of five regions of the PB (frontal lobe, parietal lobe, temporal lobe, occipital lobe, and hypothalamus) and identified 21 cell subpopulations. The cross-species comparison of mouse and pig hypothalamus revealed the shared and specific gene expression patterns at the single-cell resolution. Furthermore, we identified cell types and molecular pathways closely associated with neurological disorders, bridging the gap between gene mutations and pathogenesis. We reported, to our knowledge, the first single-cell atlas of domestic pig cerebral cortex and hypothalamus combined with a comprehensive analysis across species, providing extensive resources for future research regarding neural science, evolutionary developmental biology, and regenerative medicine.",

keywords = "Domestic pig, Brain cell atlas, Single-cell transcriptome, Neural science",

author = "Jiacheng Zhu and Fang Chen and Lihua Luo and Weiying Wu and Jinxia Dai and Jixing Zhong and Xiumei Lin and Chaochao Chai and Peiwen Ding and Langchao Liang and Shiyou Wang and Xiangning Ding and Yin Chen and Haoyu Wang and Jiaying Qiu and Feiyue Wang and Chengcheng Sun and Yuying Zeng and Jian Fang and Xiaosen Jiang and Ping Liu and Gen Tang and Xin Qiu and Xingliang Zhang and Yetian Ruan and Sanjie Jiang and Jiankang Li and Shida Zhu and Xun Xu and Fang Li and Zhongmin Liu and Gang Cao and Dongsheng Chen",

note = "Publisher Copyright: {\textcopyright} 2021 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved. Funding Information: We are thankful to the production team of China National GeneBank, Shenzhen, China. This work was supported by the China Postdoctoral Science Foundation (2017M622795), the Science, Technology and Innovation Commission of Shenzhen Municipality (JCYJ20180507183628543), and the Fundamental Research Funds for the Central Universities (2662018PY025 and 2662017PY105).",

year = "2021",

month = jul,

day = "30",

doi = "10.1016/j.scib.2021.04.002",

language = "English",

volume = "66",

pages = "1448--1461",

journal = "Science Bulletin",

issn = "2095-9273",

publisher = "Elsevier B.V.",

number = "14",

}

Zhu, J, Chen, F, Luo, L, Wu, W, Dai, J, Zhong, J, Lin, X, Chai, C, Ding, P, Liang, L, Wang, S, Ding, X, Chen, Y, Wang, H, Qiu, J, Wang, F, Sun, C, Zeng, Y, Fang, J, Jiang, X, Liu, P, Tang, G, Qiu, X, Zhang, X, Ruan, Y, Jiang, S, Li, J, Zhu, S, Xu, X, Li, F, Liu, Z, Cao, G & Chen, D 2021, 'Single-cell atlas of domestic pig cerebral cortex and hypothalamus', Science Bulletin, vol. 66, no. 14, pp. 1448-1461. https://doi.org/10.1016/j.scib.2021.04.002

TY - JOUR

T1 - Single-cell atlas of domestic pig cerebral cortex and hypothalamus

AU - Zhu, Jiacheng

AU - Chen, Fang

AU - Luo, Lihua

AU - Wu, Weiying

AU - Dai, Jinxia

AU - Zhong, Jixing

AU - Lin, Xiumei

AU - Chai, Chaochao

AU - Ding, Peiwen

AU - Liang, Langchao

AU - Wang, Shiyou

AU - Ding, Xiangning

AU - Chen, Yin

AU - Wang, Haoyu

AU - Qiu, Jiaying

AU - Wang, Feiyue

AU - Sun, Chengcheng

AU - Zeng, Yuying

AU - Fang, Jian

AU - Jiang, Xiaosen

AU - Liu, Ping

AU - Tang, Gen

AU - Qiu, Xin

AU - Zhang, Xingliang

AU - Ruan, Yetian

AU - Jiang, Sanjie

AU - Li, Jiankang

AU - Zhu, Shida

AU - Xu, Xun

AU - Li, Fang

AU - Liu, Zhongmin

AU - Cao, Gang

AU - Chen, Dongsheng

N1 - Publisher Copyright: © 2021 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved. Funding Information: We are thankful to the production team of China National GeneBank, Shenzhen, China. This work was supported by the China Postdoctoral Science Foundation (2017M622795), the Science, Technology and Innovation Commission of Shenzhen Municipality (JCYJ20180507183628543), and the Fundamental Research Funds for the Central Universities (2662018PY025 and 2662017PY105).

PY - 2021/7/30

Y1 - 2021/7/30

N2 - The brain of the domestic pig (Sus scrofa domesticus) has drawn considerable attention due to its high similarities to that of humans. However, the cellular compositions of the pig brain (PB) remain elusive. Here we investigated the single-nucleus transcriptomic profiles of five regions of the PB (frontal lobe, parietal lobe, temporal lobe, occipital lobe, and hypothalamus) and identified 21 cell subpopulations. The cross-species comparison of mouse and pig hypothalamus revealed the shared and specific gene expression patterns at the single-cell resolution. Furthermore, we identified cell types and molecular pathways closely associated with neurological disorders, bridging the gap between gene mutations and pathogenesis. We reported, to our knowledge, the first single-cell atlas of domestic pig cerebral cortex and hypothalamus combined with a comprehensive analysis across species, providing extensive resources for future research regarding neural science, evolutionary developmental biology, and regenerative medicine.

AB - The brain of the domestic pig (Sus scrofa domesticus) has drawn considerable attention due to its high similarities to that of humans. However, the cellular compositions of the pig brain (PB) remain elusive. Here we investigated the single-nucleus transcriptomic profiles of five regions of the PB (frontal lobe, parietal lobe, temporal lobe, occipital lobe, and hypothalamus) and identified 21 cell subpopulations. The cross-species comparison of mouse and pig hypothalamus revealed the shared and specific gene expression patterns at the single-cell resolution. Furthermore, we identified cell types and molecular pathways closely associated with neurological disorders, bridging the gap between gene mutations and pathogenesis. We reported, to our knowledge, the first single-cell atlas of domestic pig cerebral cortex and hypothalamus combined with a comprehensive analysis across species, providing extensive resources for future research regarding neural science, evolutionary developmental biology, and regenerative medicine.

KW - Domestic pig

KW - Brain cell atlas

KW - Single-cell transcriptome

KW - Neural science

UR - https://www.scopus.com/pages/publications/85105039594

U2 - 10.1016/j.scib.2021.04.002

DO - 10.1016/j.scib.2021.04.002

M3 - Journal article

AN - SCOPUS:85105039594

SN - 2095-9273

VL - 66

SP - 1448

EP - 1461

JO - Science Bulletin

JF - Science Bulletin

IS - 14

ER -

Single-cell atlas of domestic pig cerebral cortex and hypothalamus

Abstract

UN SDGs

User-Defined Keywords

Access to Document

Other files and links

Fingerprint

Cite this