液相色谱-电喷雾离子阱质谱结合"N in 1"模式用于高通量分析药物及其代谢物

Translated title of the contribution: Simultaneous quantitative “N in 1” analysis of drugs and their metabolites by liquid chromatography-electrospray ion trap mass spectrometry

陈学国, 赖永权, 蔡宗苇

Research output: Contribution to journalArticle

Abstract

本文建立了一种基于液相色谱-电喷雾离子阱质谱(LC-ESI-ITMS)技术和“N in 1”模式的药物及其代谢物快速高通量分离与鉴定方法. 该方法首先利用体外代谢方法得到肝微粒体中药物与各自代谢产物混合体系, 然后采用“N in 1”模式对该混合体系进行处理, 并利用 LC-ESI-ITMS 联用技术对各待测药物及其代谢物进行分析, 得到药物和代谢物的分子量信息, 进一步结合多级串联质谱(LC-MSn)分析结果获得结构信息, 对代谢物进行鉴定, 从而快速的推测代谢物结构, 进而得到药物的生物转化规律. 本方法用于6种药物及其4种代谢物的分析, 实验结果表明: 该方法具有快速、高效、灵敏, 选择性好等特点.
Translated title of the contributionSimultaneous quantitative “N in 1” analysis of drugs and their metabolites by liquid chromatography-electrospray ion trap mass spectrometry
Original languageChinese (Simplified)
Pages (from-to)757-761
Number of pages5
Journal中国科学: 化学
Volume40
Issue number6
DOIs
Publication statusPublished - Jun 2010

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