Simultaneous quantification of five major constituents in stems of Dracaena plants and related medicinal preparations from China and Vietnam by HPLC-DAD

Lan Lan Fan, Peng Fei Tu, Hubiao CHEN*, Shao Qing Cai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

A high-performance liquid chromatographic (HPLC) method was developed for the simultaneous quantification of five major bioactive constituents in the stems of resiniferous Dracaena plants from China and Vietnam, as well as those in the related traditional Chinese medicinal preparations. A diode array detector with the wavelength of 330 nm was used to monitor resveratrol, 7,4′-dihydroxyflavone and pterostilbene, while loureirin A and loureirin B were monitored at 280 nm. The five constituents were separated on an Agela SB C18 column by gradient elution using 0.008% (v/v) formic acid solution (A) and acetonitrile (B) as the mobile phase. The validation of the method included recovery, linearity, accuracy and precision (intra- and inter-day variation). The range of recoveries of this method was 98.1-104.9%, with all the constituents showing good linearity (r2 > 0.9999). The accuracy and precision were satisfactory, with the overall intra- and inter-day variation being less than 4%. The present method has been successfully applied for the determination of all five constituents in 21 related herbal samples including 10 D. cochinchinensis stem samples, seven D. cambodiana stem samples and four purchased medicinal preparations. The contents of these constituents were analyzed using principal component analysis, which can efficiently identify raw herb of Dracaena from different sources. The study may be considered helpful to the quality control of Dracena plants and its medicinal preparations.

Original languageEnglish
Pages (from-to)1191-1200
Number of pages10
JournalBiomedical Chromatography
Volume23
Issue number11
DOIs
Publication statusPublished - 2009

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

User-Defined Keywords

  • D. cambodiana
  • Dracaena cochinchinensis
  • High-performance liquid chromatography (HPLC)
  • Longxuejie
  • Principal component analysis

Fingerprint

Dive into the research topics of 'Simultaneous quantification of five major constituents in stems of Dracaena plants and related medicinal preparations from China and Vietnam by HPLC-DAD'. Together they form a unique fingerprint.

Cite this