Simultaneous quantification of eight bioactive components of Houttuynia cordata and related Saururaceae medicinal plants by on-line high performance liquid chromatography-diode array detector-electrospray mass spectrometry

Jiang Meng, Kelvin S Y LEUNG, Xiao ping Dong, Yi sheng Zhou, Zhi Hong JIANG, Zhongzhen ZHAO*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

An on-line high performance liquid chromatography (HPLC)-diode array detector (DAD)-electrospray ionization mass spectrometry (ESI-MS) method has been developed to quantify simultaneously eight bioactive chemical components in Houttuynia cordata Thunb and related Saururaceae medicinal plants. Simultaneous separation of these eight compounds was achieved on a C18 analytical column with gradient elution of acetonitrile and 0.2% acetic acid (v/v) at a flow rate of 0.6 mL/min and being detected at 280 nm. These eight compounds were completely separated within 90 min. Good linear regression relationship (r2 > 0.9978) within test ranges was shown in all calibration curves. Good repeatabilty for the quantification of these eight compounds in H. cordata was also demonstrated in this method, with intra- and inter-day variations less than 3.0%. The method established was successfully applied to quantify eight bioactive compounds in closely related species of H. cordata, which provides a new basis for quality assessment of H. cordata.

Original languageEnglish
Pages (from-to)468-474
Number of pages7
JournalFitoterapia
Volume80
Issue number8
DOIs
Publication statusPublished - Dec 2009

Scopus Subject Areas

  • Pharmacology
  • Drug Discovery

User-Defined Keywords

  • Active components
  • Houttuynia cordata Thunb
  • HPLC-DAD-ESI-MS
  • Quantitative analysis
  • Related species

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