Simultaneous determination of Ziagen and its phosphorylated metabolites by ion-pairing high-performance liquid chromatography-tandem mass spectrometry

Eliza N. Fung, Zongwei CAI*, Thimysta C. Burnette, Achintya K. Sinhababu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)

Abstract

An ion-paring HPLC-MS-MS method with positive ion mode electrospray ionization has been developed to simultaneously quantify Ziagen, carbovir monophosphate, carbovir diphosphate and carbovir triphosphate. N′,N′-Dimethylhexylamine was used as the ion-pairing agent. The presence of this ion-pairing agent allowed the retention and separation of the four compounds on a reversed-phase HPLC column as well as the detection of the nucleotides with positive ion mode electrospray ionization. The limits of detection were found to be better than 25 nM for all the analytes. Calibration curves of the analytes showed excellent linearity over the range of 25 nM to 5 μM. The relative standard deviations and accuracies for replicate analyses of quality control samples were less than 15%. The method has been successfully applied to the analysis of these compounds in human liver cells treated with Ziagen.

Original languageEnglish
Pages (from-to)285-295
Number of pages11
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume754
Issue number2
DOIs
Publication statusPublished - 25 Apr 2001

Scopus Subject Areas

  • Chemistry(all)

User-Defined Keywords

  • Carbovir phosphate
  • Ziagen

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