Simultaneous determination of Ziagen and its phosphorylated metabolites by ion-pairing high-performance liquid chromatography-tandem mass spectrometry

Eliza N. Fung; Zongwei Cai; Thimysta C. Burnette; Achintya K. Sinhababu

doi:10.1016/S0378-4347(00)00619-8

Simultaneous determination of Ziagen and its phosphorylated metabolites by ion-pairing high-performance liquid chromatography-tandem mass spectrometry

Eliza N. Fung, Zongwei Cai^*, Thimysta C. Burnette, Achintya K. Sinhababu

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

80 Citations (Scopus)

Abstract

An ion-paring HPLC-MS-MS method with positive ion mode electrospray ionization has been developed to simultaneously quantify Ziagen, carbovir monophosphate, carbovir diphosphate and carbovir triphosphate. N′,N′-Dimethylhexylamine was used as the ion-pairing agent. The presence of this ion-pairing agent allowed the retention and separation of the four compounds on a reversed-phase HPLC column as well as the detection of the nucleotides with positive ion mode electrospray ionization. The limits of detection were found to be better than 25 nM for all the analytes. Calibration curves of the analytes showed excellent linearity over the range of 25 nM to 5 μM. The relative standard deviations and accuracies for replicate analyses of quality control samples were less than 15%. The method has been successfully applied to the analysis of these compounds in human liver cells treated with Ziagen.

Original language	English
Pages (from-to)	285-295
Number of pages	11
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	754
Issue number	2
DOIs	https://doi.org/10.1016/S0378-4347(00)00619-8
Publication status	Published - 25 Apr 2001

Scopus Subject Areas

Chemistry(all)

User-Defined Keywords

Carbovir phosphate
Ziagen

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10.1016/S0378-4347(00)00619-8

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title = "Simultaneous determination of Ziagen and its phosphorylated metabolites by ion-pairing high-performance liquid chromatography-tandem mass spectrometry",

abstract = "An ion-paring HPLC-MS-MS method with positive ion mode electrospray ionization has been developed to simultaneously quantify Ziagen, carbovir monophosphate, carbovir diphosphate and carbovir triphosphate. N′,N′-Dimethylhexylamine was used as the ion-pairing agent. The presence of this ion-pairing agent allowed the retention and separation of the four compounds on a reversed-phase HPLC column as well as the detection of the nucleotides with positive ion mode electrospray ionization. The limits of detection were found to be better than 25 nM for all the analytes. Calibration curves of the analytes showed excellent linearity over the range of 25 nM to 5 μM. The relative standard deviations and accuracies for replicate analyses of quality control samples were less than 15%. The method has been successfully applied to the analysis of these compounds in human liver cells treated with Ziagen.",

keywords = "Carbovir phosphate, Ziagen",

author = "Fung, {Eliza N.} and Zongwei Cai and Burnette, {Thimysta C.} and Sinhababu, {Achintya K.}",

year = "2001",

month = apr,

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doi = "10.1016/S0378-4347(00)00619-8",

language = "English",

volume = "754",

pages = "285--295",

journal = "Journal of Chromatography B: Biomedical Sciences and Applications",

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publisher = "Elsevier BV",

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}

Simultaneous determination of Ziagen and its phosphorylated metabolites by ion-pairing high-performance liquid chromatography-tandem mass spectrometry. / Fung, Eliza N.; Cai, Zongwei; Burnette, Thimysta C. et al.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 754, No. 2, 25.04.2001, p. 285-295.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Simultaneous determination of Ziagen and its phosphorylated metabolites by ion-pairing high-performance liquid chromatography-tandem mass spectrometry

AU - Fung, Eliza N.

AU - Cai, Zongwei

AU - Burnette, Thimysta C.

AU - Sinhababu, Achintya K.

PY - 2001/4/25

Y1 - 2001/4/25

N2 - An ion-paring HPLC-MS-MS method with positive ion mode electrospray ionization has been developed to simultaneously quantify Ziagen, carbovir monophosphate, carbovir diphosphate and carbovir triphosphate. N′,N′-Dimethylhexylamine was used as the ion-pairing agent. The presence of this ion-pairing agent allowed the retention and separation of the four compounds on a reversed-phase HPLC column as well as the detection of the nucleotides with positive ion mode electrospray ionization. The limits of detection were found to be better than 25 nM for all the analytes. Calibration curves of the analytes showed excellent linearity over the range of 25 nM to 5 μM. The relative standard deviations and accuracies for replicate analyses of quality control samples were less than 15%. The method has been successfully applied to the analysis of these compounds in human liver cells treated with Ziagen.

AB - An ion-paring HPLC-MS-MS method with positive ion mode electrospray ionization has been developed to simultaneously quantify Ziagen, carbovir monophosphate, carbovir diphosphate and carbovir triphosphate. N′,N′-Dimethylhexylamine was used as the ion-pairing agent. The presence of this ion-pairing agent allowed the retention and separation of the four compounds on a reversed-phase HPLC column as well as the detection of the nucleotides with positive ion mode electrospray ionization. The limits of detection were found to be better than 25 nM for all the analytes. Calibration curves of the analytes showed excellent linearity over the range of 25 nM to 5 μM. The relative standard deviations and accuracies for replicate analyses of quality control samples were less than 15%. The method has been successfully applied to the analysis of these compounds in human liver cells treated with Ziagen.

KW - Carbovir phosphate

KW - Ziagen

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M3 - Journal article

C2 - 11339272

AN - SCOPUS:0035946719

SN - 1387-2273

VL - 754

SP - 285

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JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

IS - 2

ER -

Simultaneous determination of Ziagen and its phosphorylated metabolites by ion-pairing high-performance liquid chromatography-tandem mass spectrometry

Abstract

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