Simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high-performance liquid chromatography with fluorescence detection

Hai Tao Lu, Yue Jiang*, Hua Bin Li, Feng Chen, Ming Hung WONG

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

A simple, rapid and sensitive high-performance liquid chromatographic method with fluorescence detection for the simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline was developed, and successfully applied to the analysis of commercial tetracycline antibiotics. The separation was performed on a reverse-phase C18 column with a gradient elution composed of methanol and sodium acetate buffer (containing disodium ethylenediaminetetraacetate and calcium chloride, pH 8.10) as the mobile phase, and fluorescence detection at 532 nm (excitation at 380 nm). The detection limits for oxytetracycline, doxycycline, tetracycline and chlortetracycline were 0.1, 0.5, 0.3 and 0.4 μg L-1, respectively. Data with respect to precision and accuracy were reported and discussed.

Original languageEnglish
Pages (from-to)259-264
Number of pages6
JournalChromatographia
Volume60
Issue number5-6
DOIs
Publication statusPublished - Sep 2004

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

User-Defined Keywords

  • Column liquid chromatography
  • Fluorescence detection
  • Pharmaceuticals
  • Tetracycline antibiotics

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