TY - JOUR
T1 - Simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high-performance liquid chromatography with fluorescence detection
AU - Lu, Hai Tao
AU - Jiang, Yue
AU - Li, Hua Bin
AU - Chen, Feng
AU - WONG, Ming Hung
N1 - Funding Information:
This research was supported by the FRG (Faculty Research Grant) of Hong Kong Baptist University, the Outstanding Young Researcher Award of the Uni versity of Hong Kong and DAAD-RGC Hong Kong Academic Link.
PY - 2004/9
Y1 - 2004/9
N2 - A simple, rapid and sensitive high-performance liquid chromatographic method with fluorescence detection for the simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline was developed, and successfully applied to the analysis of commercial tetracycline antibiotics. The separation was performed on a reverse-phase C18 column with a gradient elution composed of methanol and sodium acetate buffer (containing disodium ethylenediaminetetraacetate and calcium chloride, pH 8.10) as the mobile phase, and fluorescence detection at 532 nm (excitation at 380 nm). The detection limits for oxytetracycline, doxycycline, tetracycline and chlortetracycline were 0.1, 0.5, 0.3 and 0.4 μg L-1, respectively. Data with respect to precision and accuracy were reported and discussed.
AB - A simple, rapid and sensitive high-performance liquid chromatographic method with fluorescence detection for the simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline was developed, and successfully applied to the analysis of commercial tetracycline antibiotics. The separation was performed on a reverse-phase C18 column with a gradient elution composed of methanol and sodium acetate buffer (containing disodium ethylenediaminetetraacetate and calcium chloride, pH 8.10) as the mobile phase, and fluorescence detection at 532 nm (excitation at 380 nm). The detection limits for oxytetracycline, doxycycline, tetracycline and chlortetracycline were 0.1, 0.5, 0.3 and 0.4 μg L-1, respectively. Data with respect to precision and accuracy were reported and discussed.
KW - Column liquid chromatography
KW - Fluorescence detection
KW - Pharmaceuticals
KW - Tetracycline antibiotics
UR - http://www.scopus.com/inward/record.url?scp=4744362375&partnerID=8YFLogxK
U2 - 10.1365/s10337-004-0356-z
DO - 10.1365/s10337-004-0356-z
M3 - Journal article
AN - SCOPUS:4744362375
SN - 0009-5893
VL - 60
SP - 259
EP - 264
JO - Chromatographia
JF - Chromatographia
IS - 5-6
ER -