Simultaneous determination of multiple marker constituents in concentrated Gegen Tang granule by high performance liquid chromatography

Jingzheng Song, Quanbin Han, Chunfeng Qiao, Yuekeung Yip, Hongxi Xu*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

15 Citations (Scopus)


Background: Concentrated Gegen Tang (GT) granule is a widely available traditional Chinese medicinal product for the treatment of cold and flu. There was no reliable analytical method available for the quality assessment of GT granules. Methods: An HPLC method with an Agilent Zorbax SB-Phenyl Stablebond column (250 × 4.6 mm, 5 μm) was developed and validated. The mobile phase gradient was a mixture of 0.1% trifluoroacetic acid (TFA) in acetonitrile (ACN) and 0.1% TFA in water. The detection with a diode-array detector was set at 207, 230, 250 and 275 nm. Seven components, namely puerarin, daidzein, paeoniflorin, cinnamic acid, glycyrrhizin, ephedrine and pseudoephedrine were selected as marker compounds for the evaluation. Results: The regression equations revealed good linear relationships (correlation coefficients: 0.9994-0.9998) between the peak areas and concentrations. The recovery was between 98.8% and 101.7% with good precision and accuracy. The quality of GT granule from four different manufacturers was evaluated with this newly developed method. Samples from four manufacturers showed similar profiles but contents of the detected markers varied significantly among manufacturers and batches. Conclusion: A new method using high performance liquid chromatography (HPLC) has been developed for simple and reliable quality control of commercial concentrated GT granules. Sensitivity was increased by multi-wavelength detection. The contents of selected marker components in GT granules varied significantly among manufacturers and batches, making it necessary to evaluate the quality of concentrated GT granules in the market.

Original languageEnglish
Article number7
Number of pages8
JournalChinese Medicine
Publication statusPublished - 20 Jun 2007

Scopus Subject Areas

  • Pharmacology
  • Complementary and alternative medicine

User-Defined Keywords

  • High Performance Liquid Chromatography
  • Cinnamic Acid
  • Trigeminal Neuralgia
  • Ephedrine
  • Pseudoephedrine


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