Simultaneous determination of monophenyltin (MPT), diphenyltin (DPT) and triphenyltin (TPT) by a UV detector was developed using high performance liquid chromatography (HPLC) with methanol-acetic acid-water mixtures containing thiol compounds as mobile phase. Peaks of TPT, DPT and MPT were completely separated and they were detected using UV detector at 257 nm. The effects of thiol compounds, i.e. dithiothreitol (DTT), dithioerythritol (DTE) and 2-mercapoethanol (ME) on the peak heights and retention times of phenyltin compounds were investigated, and the optimum performance was achieved with methanol-acetic acid-water (60:10:30 [v/v/v]) containing 5 mM DTE. The different affinity of thiol group with different phenyltins was found helpful to the separation of phenyltins. The developed method was successfully applied to the analysis of phenyltins in organotin biodegradation by Pseudomonas aeruginosa.
|Number of pages||7|
|Journal||Pakistan Journal of Biotechnology|
|Publication status||Published - 25 Jul 2006|