Simultaneous determination of glutamate and aspartate in rat periaqueductal gray matter microdialysates by capillary electrophoresis with laser-induced fluorescence

Hong Li Chen; Xiao Jun Zhang; Sheng Da Qi; Hong Xi Xu; Joseph J.Y. Sung; Zhaoxiang BIAN

doi:10.1016/j.jchromb.2009.08.006

Simultaneous determination of glutamate and aspartate in rat periaqueductal gray matter microdialysates by capillary electrophoresis with laser-induced fluorescence

Hong Li Chen, Xiao Jun Zhang, Sheng Da Qi, Hong Xi Xu, Joseph J.Y. Sung, Zhaoxiang BIAN^*

^*Corresponding author for this work

School of Chinese Medicine

Research output: Contribution to journal › Journal article › peer-review

14 Citations (Scopus)

Abstract

This study presented a capillary zone electrophoresis (CZE) method for the analysis of trace amount of neurotransmitters glutamate (Glu) and aspartate (Asp) in the microdialysates of rat periaqueductal gray matter (PAG). Glu and Asp were derivatized with the fluorescent agent 5-carboxyfluorescein N-succinimidyl ester (CFSE) for the first time and detected by laser-induced fluorescence (LIF) after separation by CZE. The concentration detection limits (S/N = 2) were 6.9 × 10^-10 M and 8.1 × 10^-10 M for Glu and Asp, respectively. The repeatability (expressed as RSD) of the migration times of CFSE-amino acid were better than 0.5%, and not higher than 1.5% even over the period of 1 month. This method was applied to quantify Glu and Asp in rat PAG microdialysates with the treatment of formalin injection, and the measured basal concentrations of Glu and Asp were (22.4 ± 1.6) × 10^-6 M and (0.9 ± 0.1) × 10^-6 M, respectively.

Original language	English
Pages (from-to)	3248-3252
Number of pages	5
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	877
Issue number	27
DOIs	https://doi.org/10.1016/j.jchromb.2009.08.006
Publication status	Published - 1 Oct 2009

Scopus Subject Areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

User-Defined Keywords

5-Carboxyfluorescein N-succinimidyl ester
Aspartate
Capillary electrophoresis
Glutamate
Laser-induced fluorescence
Microdialysis

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10.1016/j.jchromb.2009.08.006

Cite this

Chen, H. L., Zhang, X. J., Qi, S. D., Xu, H. X., Sung, J. J. Y., & BIAN, Z. (2009). Simultaneous determination of glutamate and aspartate in rat periaqueductal gray matter microdialysates by capillary electrophoresis with laser-induced fluorescence. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 877(27), 3248-3252. https://doi.org/10.1016/j.jchromb.2009.08.006

@article{bf8b7630c162430aaed7a34803dc8ef3,

title = "Simultaneous determination of glutamate and aspartate in rat periaqueductal gray matter microdialysates by capillary electrophoresis with laser-induced fluorescence",

abstract = "This study presented a capillary zone electrophoresis (CZE) method for the analysis of trace amount of neurotransmitters glutamate (Glu) and aspartate (Asp) in the microdialysates of rat periaqueductal gray matter (PAG). Glu and Asp were derivatized with the fluorescent agent 5-carboxyfluorescein N-succinimidyl ester (CFSE) for the first time and detected by laser-induced fluorescence (LIF) after separation by CZE. The concentration detection limits (S/N = 2) were 6.9 × 10-10 M and 8.1 × 10-10 M for Glu and Asp, respectively. The repeatability (expressed as RSD) of the migration times of CFSE-amino acid were better than 0.5%, and not higher than 1.5% even over the period of 1 month. This method was applied to quantify Glu and Asp in rat PAG microdialysates with the treatment of formalin injection, and the measured basal concentrations of Glu and Asp were (22.4 ± 1.6) × 10-6 M and (0.9 ± 0.1) × 10-6 M, respectively.",

keywords = "5-Carboxyfluorescein N-succinimidyl ester, Aspartate, Capillary electrophoresis, Glutamate, Laser-induced fluorescence, Microdialysis",

author = "Chen, {Hong Li} and Zhang, {Xiao Jun} and Qi, {Sheng Da} and Xu, {Hong Xi} and Sung, {Joseph J.Y.} and Zhaoxiang BIAN",

note = "Funding Information: Financial support from Hong Kong Jockey Club Institute of Chinese Medicine (JCICM-4-07) is gratefully acknowledged.",

year = "2009",

month = oct,

day = "1",

doi = "10.1016/j.jchromb.2009.08.006",

language = "English",

volume = "877",

pages = "3248--3252",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "Elsevier",

number = "27",

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Chen, HL, Zhang, XJ, Qi, SD, Xu, HX, Sung, JJY & BIAN, Z 2009, 'Simultaneous determination of glutamate and aspartate in rat periaqueductal gray matter microdialysates by capillary electrophoresis with laser-induced fluorescence', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 877, no. 27, pp. 3248-3252. https://doi.org/10.1016/j.jchromb.2009.08.006

Simultaneous determination of glutamate and aspartate in rat periaqueductal gray matter microdialysates by capillary electrophoresis with laser-induced fluorescence. / Chen, Hong Li; Zhang, Xiao Jun; Qi, Sheng Da et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 877, No. 27, 01.10.2009, p. 3248-3252.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Simultaneous determination of glutamate and aspartate in rat periaqueductal gray matter microdialysates by capillary electrophoresis with laser-induced fluorescence

AU - Chen, Hong Li

AU - Zhang, Xiao Jun

AU - Qi, Sheng Da

AU - Xu, Hong Xi

AU - Sung, Joseph J.Y.

AU - BIAN, Zhaoxiang

N1 - Funding Information: Financial support from Hong Kong Jockey Club Institute of Chinese Medicine (JCICM-4-07) is gratefully acknowledged.

PY - 2009/10/1

Y1 - 2009/10/1

N2 - This study presented a capillary zone electrophoresis (CZE) method for the analysis of trace amount of neurotransmitters glutamate (Glu) and aspartate (Asp) in the microdialysates of rat periaqueductal gray matter (PAG). Glu and Asp were derivatized with the fluorescent agent 5-carboxyfluorescein N-succinimidyl ester (CFSE) for the first time and detected by laser-induced fluorescence (LIF) after separation by CZE. The concentration detection limits (S/N = 2) were 6.9 × 10-10 M and 8.1 × 10-10 M for Glu and Asp, respectively. The repeatability (expressed as RSD) of the migration times of CFSE-amino acid were better than 0.5%, and not higher than 1.5% even over the period of 1 month. This method was applied to quantify Glu and Asp in rat PAG microdialysates with the treatment of formalin injection, and the measured basal concentrations of Glu and Asp were (22.4 ± 1.6) × 10-6 M and (0.9 ± 0.1) × 10-6 M, respectively.

AB - This study presented a capillary zone electrophoresis (CZE) method for the analysis of trace amount of neurotransmitters glutamate (Glu) and aspartate (Asp) in the microdialysates of rat periaqueductal gray matter (PAG). Glu and Asp were derivatized with the fluorescent agent 5-carboxyfluorescein N-succinimidyl ester (CFSE) for the first time and detected by laser-induced fluorescence (LIF) after separation by CZE. The concentration detection limits (S/N = 2) were 6.9 × 10-10 M and 8.1 × 10-10 M for Glu and Asp, respectively. The repeatability (expressed as RSD) of the migration times of CFSE-amino acid were better than 0.5%, and not higher than 1.5% even over the period of 1 month. This method was applied to quantify Glu and Asp in rat PAG microdialysates with the treatment of formalin injection, and the measured basal concentrations of Glu and Asp were (22.4 ± 1.6) × 10-6 M and (0.9 ± 0.1) × 10-6 M, respectively.

KW - 5-Carboxyfluorescein N-succinimidyl ester

KW - Aspartate

KW - Capillary electrophoresis

KW - Glutamate

KW - Laser-induced fluorescence

KW - Microdialysis

UR - http://www.scopus.com/inward/record.url?scp=69749114291&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2009.08.006

DO - 10.1016/j.jchromb.2009.08.006

M3 - Journal article

C2 - 19700376

AN - SCOPUS:69749114291

SN - 1570-0232

VL - 877

SP - 3248

EP - 3252

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 27

ER -

Chen HL, Zhang XJ, Qi SD, Xu HX, Sung JJY, BIAN Z. Simultaneous determination of glutamate and aspartate in rat periaqueductal gray matter microdialysates by capillary electrophoresis with laser-induced fluorescence. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2009 Oct 1;877(27):3248-3252. doi: 10.1016/j.jchromb.2009.08.006

Simultaneous determination of glutamate and aspartate in rat periaqueductal gray matter microdialysates by capillary electrophoresis with laser-induced fluorescence

Abstract

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