Simultaneous determination of amino acids, purines and derivatives in serum by ultrahigh-performance liquid chromatography/tandem mass spectrometry

Yuanyuan Zheng, Xiaona Li, Xiangfeng Chen, Zongwei CAI*, Hongzhi Zhao

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Rationale: Amino acids, purines and derivatives play a key role in physiological and pathological processes, such as the development of gestational diabetes mellitus. However, little literature has reported the simultaneous quantification of amino acids and purines. Therefore, a reliable and robust method for the determination of amino acids and purines is necessary. Methods: A rapid and simple method based on ultrahigh-performance liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS) was developed to determine the concentrations of twenty amino acids or derivatives and three purines or derivatives in serum. Results: The method was validated for linearity of calibration curve, limit of detection, limit of quantification, inter-day and intra-day precision, recovery, stability and matrix effect. The correlation coefficients of calibration curves were higher than 0.993 except for isoleucine. The recoveries ranged from 78.4% to 128.0% for three different concentrations of the spiked analytes. The matrix effect value ranged from −7.3% to 9.2%. Conclusions: A rapid and simple UHPLC/MS/MS method for the simultaneous measurement of amino acids, purines and derivatives in serum samples was developed. The method was successfully applied to determine and compare the concentrations of analytes between gestational diabetes mellitus samples and controls.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalRapid Communications in Mass Spectrometry
Volume33
Issue number1
DOIs
Publication statusPublished - 15 Jan 2019

Scopus Subject Areas

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

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