Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activity

Chung Hang Leung; Daniel Shiu Hin Chan; Bradley Yat Wah Man; Chuan Jen Wang; Wing Lam; Yung Chi Cheng; David W F FONG; Wendy W L HSIAO; Edmond Dik Lung MA

doi:10.1021/ac1025896

Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activity

Chung Hang Leung^*
, Daniel Shiu Hin Chan
, Bradley Yat Wah Man
, Chuan Jen Wang
, Wing Lam
, Yung Chi Cheng
, David W F FONG
, Wendy W L HSIAO
, Edmond Dik Lung MA

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

103 Citations (Scopus)

Abstract

A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.

Original language	English
Pages (from-to)	463-466
Number of pages	4
Journal	Analytical Chemistry
Volume	83
Issue number	2
DOIs	https://doi.org/10.1021/ac1025896
Publication status	Published - 15 Jan 2011

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10.1021/ac1025896

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title = "Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activity",

abstract = "A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.",

author = "Leung, \{Chung Hang\} and Chan, \{Daniel Shiu Hin\} and Man, \{Bradley Yat Wah\} and Wang, \{Chuan Jen\} and Wing Lam and Cheng, \{Yung Chi\} and FONG, \{David W F\} and HSIAO, \{Wendy W L\} and MA, \{Edmond Dik Lung\}",

note = "This work was supported by the Hong Kong Baptist University (FRG1/10-11/008 and FRG2/09-10/070) and the National Institutes of Health U.S.A. (CA-063477 and AI038204). Y.-C. Cheng is a Fellow of the National Foundation for Cancer Research. Part of C.-H. Leung{\textquoteright}s salary is supported by the Research Grants Council of Hong Kong HKBU 2/07C to WLWH.",

year = "2011",

month = jan,

day = "15",

doi = "10.1021/ac1025896",

language = "English",

volume = "83",

pages = "463--466",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activity

AU - Leung, Chung Hang

AU - Chan, Daniel Shiu Hin

AU - Man, Bradley Yat Wah

AU - Wang, Chuan Jen

AU - Lam, Wing

AU - Cheng, Yung Chi

AU - FONG, David W F

AU - HSIAO, Wendy W L

AU - MA, Edmond Dik Lung

N1 - This work was supported by the Hong Kong Baptist University (FRG1/10-11/008 and FRG2/09-10/070) and the National Institutes of Health U.S.A. (CA-063477 and AI038204). Y.-C. Cheng is a Fellow of the National Foundation for Cancer Research. Part of C.-H. Leung’s salary is supported by the Research Grants Council of Hong Kong HKBU 2/07C to WLWH.

PY - 2011/1/15

Y1 - 2011/1/15

N2 - A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.

AB - A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.

UR - https://www.scopus.com/pages/publications/78751558009

U2 - 10.1021/ac1025896

DO - 10.1021/ac1025896

M3 - Journal article

C2 - 21114271

AN - SCOPUS:78751558009

SN - 0003-2700

VL - 83

SP - 463

EP - 466

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 2

ER -

Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activity

Abstract

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