Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activity

Chung Hang Leung*, Daniel Shiu Hin Chan, Bradley Yat Wah Man, Chuan Jen Wang, Wing Lam, Yung Chi Cheng, David W F FONG, Wendy W L HSIAO, Edmond Dik Lung MA

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

99 Citations (Scopus)

Abstract

A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.

Original languageEnglish
Pages (from-to)463-466
Number of pages4
JournalAnalytical Chemistry
Volume83
Issue number2
DOIs
Publication statusPublished - 15 Jan 2011

Scopus Subject Areas

  • Analytical Chemistry

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