Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activity

Chung Hang Leung; Daniel Shiu Hin Chan; Bradley Yat Wah Man; Chuan Jen Wang; Wing Lam; Yung Chi Cheng; David W F FONG; Wendy W L HSIAO; Edmond Dik Lung MA

doi:10.1021/ac1025896

Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activity

Chung Hang Leung^*, Daniel Shiu Hin Chan, Bradley Yat Wah Man, Chuan Jen Wang, Wing Lam, Yung Chi Cheng, David W F FONG, Wendy W L HSIAO, Edmond Dik Lung MA

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

101 Citations (Scopus)

Abstract

A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.

Original language	English
Pages (from-to)	463-466
Number of pages	4
Journal	Analytical Chemistry
Volume	83
Issue number	2
DOIs	https://doi.org/10.1021/ac1025896
Publication status	Published - 15 Jan 2011

Scopus Subject Areas

Analytical Chemistry

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title = "Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activity",

abstract = "A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.",

author = "Leung, {Chung Hang} and Chan, {Daniel Shiu Hin} and Man, {Bradley Yat Wah} and Wang, {Chuan Jen} and Wing Lam and Cheng, {Yung Chi} and FONG, {David W F} and HSIAO, {Wendy W L} and MA, {Edmond Dik Lung}",

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doi = "10.1021/ac1025896",

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AU - Leung, Chung Hang

AU - Chan, Daniel Shiu Hin

AU - Man, Bradley Yat Wah

AU - Wang, Chuan Jen

AU - Lam, Wing

AU - Cheng, Yung Chi

AU - FONG, David W F

AU - HSIAO, Wendy W L

AU - MA, Edmond Dik Lung

PY - 2011/1/15

Y1 - 2011/1/15

N2 - A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.

AB - A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.

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SN - 0003-2700

VL - 83

SP - 463

EP - 466

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 2

ER -

Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activity

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