@article{f85f01930a5d4e169a02e30d69a0b728,
title = "Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activity",
abstract = "A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.",
author = "Leung, {Chung Hang} and Chan, {Daniel Shiu Hin} and Man, {Bradley Yat Wah} and Wang, {Chuan Jen} and Wing Lam and Cheng, {Yung Chi} and FONG, {David W F} and HSIAO, {Wendy W L} and MA, {Edmond Dik Lung}",
note = "This work was supported by the Hong Kong Baptist University (FRG1/10-11/008 and FRG2/09-10/070) and the National Institutes of Health U.S.A. (CA-063477 and AI038204). Y.-C. Cheng is a Fellow of the National Foundation for Cancer Research. Part of C.-H. Leung{\textquoteright}s salary is supported by the Research Grants Council of Hong Kong HKBU 2/07C to WLWH.",
year = "2011",
month = jan,
day = "15",
doi = "10.1021/ac1025896",
language = "English",
volume = "83",
pages = "463--466",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "2",
}